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PKNOX2 Antibody (4B6)

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Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - PKNOX2 monoclonal antibody (M01), clone 4B6. Analysis of PKNOX2 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of monoclonal antibody to PKNOX2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi ( Cat # H00063876-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - PKNOX2 monoclonal antibody (M01), clone 4B6 Analysis of PKNOX2 expression in Hela S3 NE.
Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody (M01), clone 4B6.Lane 1: PKNOX2 transfected lysate(51.9 KDa).Lane 2: ...read more
Sandwich ELISA: PKNOX2 Antibody (4B6) [H00063876-M01] - Detection limit for recombinant GST tagged PKNOX2 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
4B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PKNOX2 Antibody (4B6) Summary

Immunogen
PKNOX2 (NP_071345, 116 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PELDNLMVKAIQVLRIHLLELEKVNELCKDFCNRYITCFKTKMHSDNLLRNDLGGPYSPNQPSINLHSQDLLQNSPNSMSGVSNNPQGIVVPASALQQGN
Specificity
PKNOX2 - PBX/knotted 1 homeobox 2
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PKNOX2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validaton and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKNOX2 Antibody (4B6)

  • homeobox protein PKNOX2
  • PBX/knFLJ13074
  • PBX/knPREP2Homeobox protein PREP-2

Background

Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain andplay fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acidloop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2within the homeodomain (Imoto et al., 2001 (PubMed 11549286)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PKNOX2
Entrez
Uniprot