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PRDM14 Antibody [Unconjugated]

Name: PRDM14 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF6175
Price: 459 USD

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Western blot shows lysates of BG01V human embryonic stem cells (undifferentiated) and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human PRDM14 Antigen ...read more

PRDM14 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, ICC/IF
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

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Catalog# & Formulation	Size	Price
Carrier Free AF6175-SP

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PRDM14 Antibody [Unconjugated] Summary

Immunogen	E. coli-derived recombinant human PRDM14 Ala2-Asp201 Accession # Q9GZV8
Specificity	Detects human PRDM14 in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	PRDM14
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry 5-15 ug/mL
Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDM14 Antibody [Unconjugated]

MGC59730
PFM11
PR domain containing 14
PR domain zinc finger protein 14
PR domain-containing protein 14
PRDM14
PR-domain zinc finger protein 14

Background

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400‑568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up‑regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PRDM14 Antibody
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AF6175

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Applications: WB, ICC/IF

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Sheep IgG Isotype Control [Unconjugated]
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Bioinformatics

Gene Symbol	PRDM14
Uniprot	Q9GZV8(Human)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

PRDM14 Antibody [Unconjugated]

Species Glossary

Images

Product Details

Order Details

PRDM14 Antibody [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for PRDM14 Antibody [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for PRDM14 Antibody (AF6175) (0)

Reviews for PRDM14 Antibody (AF6175) (0)

Product General Protocols

Video Protocols

FAQs for PRDM14 Antibody (AF6175) (0)

Secondary Antibodies

Isotype Controls

Additional PRDM14 Products

Blogs on PRDM14

Customers Who Bought This Also Bought

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Product PDFs

Calculators

Review this Product

Bioinformatics