![Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Mouse skeletal muscle lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of mAb 192 at 0.03 ug/ml before detection using an anti-mouse IgG1 secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for AChR is 58.9 kDa. Chimeric mouse igG1 version of mAb 192 successfully detected AChR in mouse skeletal muscle tissue lysate. Though this antibody is targetted to the human AChR it also reacts with mouse AChR.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-1-CHRNA1-Antibody-mAb-192-Western-Blot-NBP2-81070-img0002.jpg "Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Mouse skeletal muscle lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of mAb 192 at 0.03 ug/ml before detection using an anti-mouse IgG1 secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for AChR is 58.9 kDa. Chimeric mouse igG1 version of mAb 192 successfully detected AChR in mouse skeletal muscle tissue lysate. Though this antibody is targetted to the human AChR it also reacts with mouse AChR.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, B/N |
| Clone | mAb 192 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Human muscle AChR. |
| Specificity | The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | CHRNA1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody binds to the human muscle Acetylcholine receptor, which is a ligand-gated ion channel, transducing an action potential into the muscle cells. The antibody binds to the main immunogenic region (MIR) and prevents ligand-binding. |
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| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-81070 | Applications | Species |
|---|---|---|
| Gupta R, Johnston TR, Chen VY et al. Human Motor Endplate Survival after Chronic Peripheral Nerve Injury medRxiv : the preprint server for health sciences 2023-10-14 [PMID: 37873401] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (NBP2-81070)Find related products by research area.
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| Gene Symbol | CHRNA1 |
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of mAb 192 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81070, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-1-CHRNA1-Antibody-mAb-192-Immunocytochemistry-Immunofluorescence-NBP2-81070-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of mAb 192 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81070, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.")
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