![Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against HEK293 (1) and CHRNA5 (AA: 23-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-2D2D10-Western-Blot-NBP2-61677-img0004.jpg "Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against HEK293 (1) and CHRNA5 (AA: 23-254)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow |
| Clone | 2D2D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 5/CHRNA5 (AA: extra 23-254) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHRNA5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (NBP2-61677)Find related products by research area.
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| Gene Symbol | CHRNA5 |
![Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis of SK-N-SH cells using CHRNA5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-2D2D10-Flow-Cytometry-NBP2-61677-img0002.jpg "Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis of SK-N-SH cells using CHRNA5 mouse mAb (green) and negative control (red).")
![Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-2D2D10-Western-Blot-NBP2-61677-img0003.jpg "Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)")
![Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), membrane protein lysate of C6 (3), and C6 (4) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-2D2D10-Western-Blot-NBP2-61677-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), membrane protein lysate of C6 (3), and C6 (4) cell lysate.")
![ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-2D2D10-ELISA-NBP2-61677-img0001.jpg "ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")