![Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Western-Blot-NBP2-46603-img0005.jpg "Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | A103 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant human Melan-A/MART-1. [Uniprot# Q16655] |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
| Publications using NBP2-46603 | Applications | Species |
|---|---|---|
| Joshi P, Kooshki M, Aldrich W et al. Expression of natural killer cell regulatory microRNA by uveal melanoma cancer stem cells. Clin. Exp. Metastasis. 2016-08-26 [PMID: 27565163] (ICC/IF) | ICC/IF | |
| Baranda J, Mohyuddin G, Bur A et al. A druggable copper-signalling pathway that drives inflammation Translational Medicine Communications 2023-02-27 (IHC-P, Human) Details: Citation using the version of this antibody. |
IHC-P | Human |
| Wang Z, Li J, Gao Y et al. Transglutaminase-2 of pancreatic cancer cells assembles a CXCL12-keratin 19-coat that mediates the resistance to immunotherapy bioRxiv | ||
| Li S, Zenkel M, Kruse FE et al. Identification, Isolation, and Characterization of Melanocyte Precursor Cells in the Human Limbal Stroma International journal of molecular sciences 2022-03-29 [PMID: 35409129] (FLOW, Human) | FLOW | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Melan-A/MART-1 Antibody (NBP2-46603)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MLANA |
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells and membranous-cytoplasmic pattern in some necrotic areas.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0003.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells and membranous-cytoplasmic pattern in some necrotic areas.")
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (A103) NBP2-46603AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-BSA-Free-Flow-Cytometry-NBP2-46603-img0007.jpg "Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (A103) NBP2-46603AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells. The tumor stroma cells did not display any Melan-A/MART-1 expression signal.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0002.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells. The tumor stroma cells did not display any Melan-A/MART-1 expression signal.")
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0004.jpg "Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody [A103] NBP2-46603F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0006.jpg "Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody [A103] NBP2-46603F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")