MITF Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules. Although it has a predicted MW of 58 kDa, it runs anomalously in SDS-PAGE at 57‑66 kDa. MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression. Human MITF-A1 is 526 amino acids (aa) in length. It contains a bHLH DNA binding region (aa 309‑369) and a Leu-zipper domain (aa 374‑395). There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423. MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC. Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa 294‑299. One variant has a deletion of aa 139‑194, a second has an 11 aa substitution for aa 1‑118, and three others show variable substitutions over aa 1‑35. Over aa 119‑289, human MITF-A1 shares 96% aa identity with mouse MITF-A1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, ICC/IF, IHC-Fr.

Showing Publications 1 - 9 of 9.

Publications using AF5769	Applications	Species
N Comandante, DG Baumann, M Fallahi-Si AP-1 transcription factor network explains diverse patterns of cellular plasticity in melanoma cells Cell Reports, 2022-08-02;40(5):111147. 2022-08-02 [PMID: 35926467] (ICC/IF, Human)	ICC/IF	Human
Justine M. Renauld, Vibhuti Khan, Martín L. Basch Intermediate Cells of Dual Embryonic Origin Follow a Basal to Apical Gradient of Ingression Into the Lateral Wall of the Cochlea Frontiers in Cell and Developmental Biology 3/8/2022 [PMID: 35372344]
Sophie Colombo, Valérie Petit, Roselyne Y. Wagner, Delphine Champeval, Ichiro Yajima, Franck Gesbert et al. Stabilization of beta -catenin promotes melanocyte specification at the expense of the Schwann cell lineage Development 1/15/2022 [PMID: 34878101]
Marketa Kaucka, Bara Szarowska, Michaela Kavkova, Maria Eleni Kastriti, Polina Kameneva, Inga Schmidt et al. Nerve-associated Schwann cell precursors contribute extracutaneous melanocytes to the heart, inner ear, supraorbital locations and brain meninges Cellular and Molecular Life Sciences 8/1/2021 [PMID: 34274976]
MS Prasad, RM Charney, LJ Patel, MI García-Cas Distinct molecular profile and restricted stem cell potential defines the prospective human cranial neural crest from embryonic stem cell state Stem Cell Research, 2020-11-11;49(0):102086. 2020-11-11 [PMID: 33370869] (ICC, Human)	ICC	Human
Gustavo A. Gomez, Maneeshi S. Prasad, Man Wong, Rebekah M. Charney, Patrick B. Shelar, Nabjot Sandhu et al. WNT/ beta -catenin modulates the axial identity of embryonic stem cell-derived human neural crest Development 1/7/2019 [PMID: 31399472]
Ruth Halaban, Antonella Bacchiocchi, Robert Straub, Jian Cao, Mario Sznol, Deepak Narayan et al. A novel anti-melanoma SRC-family kinase inhibitor Oncotarget 3/19/2019 [PMID: 31040916]
Gustavo A. Gomez, Maneeshi S. Prasad, Nabjot Sandhu, Patrick B. Shelar, Alan W. Leung, Martín I. García-Castro Human neural crest induction by temporal modulation of WNT activation Developmental Biology 5/1/2019 [PMID: 30826399]
Adameyko I, Lallemend F, Furlan A, Zinin N, Aranda S, Kitambi SS, Blanchart A, Favaro R, Nicolis S, Lubke M, Muller T, Birchmeier C, Suter U, Zaitoun I, Takahashi Y, Ernfors P Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest. Development, 2012-01-01;139(2):397-410. 2012-01-01 [PMID: 22186729] (IHC-Fr, Mouse)	IHC-Fr	Mouse

Array AF5769

• MITF Antibodies
• MITF Lysate
• MITF Proteins