Ly-6G/Ly-6C Antibody (RB6-8C5) - BSA Free

The lymphocyte antigen 6 (Ly-6) complex was first described in mice and since its discovery over 20 genes related to the Ly-6 complex on chromosome 15 have been classified (1). The Ly-6 family is commonly used as a cell-surface differentiation marker on both normal and abnormal cells (2). Ly-6 complex, locus G (Ly-6G) and Ly-6 complex, locus C (Ly-6C) both belong to the murine Ly6/urokinase-type plasminogen activator receptor (uPAR) family of genes, which share a common structure (3). The Ly-6 family of proteins is characterized by a ~90 amino acid (aa) LU domain that consists of a three-finger structure formed by disulfide bonds (1,3). Additionally, many Ly6 proteins have a C-terminal GPI anchor, including Ly-6G/Ly-6C (1,3). Ly-6C protein has a molecular weight of 14 kDa and is expressed in some CD4+ and CD8+ T-cells, monocytes, neutrophils, and dendritic cells (1). Ly-6C has uniform expression in neutrophils but varies in monocytes where Ly-6Chi expression is specific for immature/inflammatory monocytes and Ly-6Clo expression identifies mature/residential monocytes (1,3). Ly-6G, on the other hand, is a protein with a molecular weight of 25 kDa with its expression uniquely restricted to neutrophils (1).

Expression of Ly-6G and Ly-6C, along with other lineage markers, help define specific cell types (2,4). For example, mouse myeloid derived suppressor cells (MDSCs), which play a role in inflammation and tumor development, are further subdivided into granulocytic (G-MDSCs) or monocytic (Mo-MDSCs) (4). Mo-MDSCs express Ly-6Chi along with CD11b, but are negative for Ly-6G, whereas G-MDSCs express Ly-6Clo/int,Ly-6Ghi , and CD11b (4).

The monoclonal antibody RB6-8C5 first identified granulocyte-differentiation antigen (Gr-1) which is primarily expressed on the surface of neutrophils and immature myeloid cells, and to a lesser extent on lymphocytes and macrophages (1). Gr-1 expression increases as neutrophils mature but remains is stably expressed in monocytes (1). The RB6-8C5 reacts with both Ly-6G and Ly-6C, whereas 1A8 antibody is specific for Ly-6G (1). Antibodies against Ly-6G, such as RB6-8C5 and 1A8, are commonly used to identify a role for neutrophils in mouse models of disease as they are efficient for quickly depleting neutrophils (1). RB6-8C5, however, also targets and depletes Ly-6Chi monocytes in addition to other Ly-6G-expressing cells (1).

References

1. Lee, P. Y., Wang, J. X., Parisini, E., Dascher, C. C., & Nigrovic, P. A. (2013). Ly6 family proteins in neutrophil biology. Journal of leukocyte biology. https://doi.org/10.1189/jlb.0113014

2. Bamezai A. (2004). Mouse Ly-6 proteins and their extended family: markers of cell differentiation and regulators of cell signaling. Archivum immunologiae et therapiae experimentalis.

3. Loughner, C. L., Bruford, E. A., McAndrews, M. S., Delp, E. E., Swamynathan, S., & Swamynathan, S. K. (2016). Organization, evolution and functions of the human and mouse Ly6/uPAR family genes. Human genomics. https://doi.org/10.1186/s40246-016-0074-2

4. Kong, Y. Y., Fuchsberger, M., Xiang, S. D., Apostolopoulos, V., & Plebanski, M. (2013). Myeloid derived suppressor cells and their role in diseases. Current medicinal chemistry. https://doi.org/10.2174/0929867311320110006

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, In Vivo, WB.

Showing Publications 1 - 10 of 25. Show All 25 Publications. Collapse Publications.

Publications using NBP2-00441	Applications	Species
Zhang K, Zhang MX, Meng XX et al. Targeting GPR65 alleviates hepatic inflammation and fibrosis by suppressing the JNK and NF-?B pathways Military Medical Research 2023-11-25 [PMID: 38001521] (IHC, WB, Human, Mouse)	IHC, WB	Human, Mouse
Tang N, Gong XR, Huang H, Meng Q Activated neutrophil-derived exosomes contribute to blood-brain barrier damage and hemorrhagic transformation after cerebral ischemia/reperfusion Brain research 2023-04-26 [PMID: 37116559] (IHC, Mouse)	IHC	Mouse
Li J, Zou X, Yang S Et al. Neutrophil Extracellular Traps Participate in the Development of Gastric Cancer Associated Thrombosis Research Square 2021-07-27 [PMID: 36051331] (ICC/IF)	ICC/IF
Ruhela D, Bhopale VM, Kalakonda S et al. Astrocyte-derived microparticles initiate a neuroinflammatory cycle due to carbon monoxide poisoning Brain Behav Immun Health 2021-12-01 [PMID: 34917988] (WB, FLOW, Mouse) Details: Citation using the PerCP format of this antibody.	WB, FLOW	Mouse
Spencer NR, Radnaa E, Baljinnyam T Et al. Development of a mouse model of ascending infection and preterm birth PLoS One 2021-12-02 [PMID: 34855804] (IHC-P, Mouse) Details: Citation using the DyLight 650 version of this antibody.	IHC-P	Mouse
Zhang S, Cao Y, Du J Et al. Neutrophil extracellular traps contribute to tissue plasminogen activator resistance in acute ischemic stroke FASEB journal : official publication of the Federation of American Societies for Experimental Biology Sep 1 2021 [PMID: 34449927] (ICC/IF, Human)	ICC/IF	Human
Keshava S, Magisetty J, Tucker TA et al. Endothelial Cell Protein C Receptor Deficiency Attenuates Streptococcus Pneumoniae-induced Pleural Fibrosis American journal of respiratory cell and molecular biology 2021-02-18 [PMID: 33600743]
Kondreddy V, Pendurthi Ur, Xu X et Al. FVIIa (Factor VIIa) Induces Biased Cytoprotective Signal in Mice Through the Cleavage of PAR (Protease-Activated Receptor)-1 at Canonical Arg41 Site Arterioscler. Thromb. Vasc. Biol. 2020-03-26 [PMID: 32212848] (Mouse)		Mouse
Zhang K, Shi Z et al. Silencing lncRNA Lfar1 alleviates the classical activation and pyoptosis of macrophage in hepatic fibrosis. Cell Death Dis 2020-02-18 [PMID: 32071306] (WB, IF/IHC, Mouse)	WB, IF/IHC	Mouse
Miyabe C, Miyabe Y, Moreno L et al. Dectin-2-induced CCL2 production in tissue-resident macrophages ignites cardiac arteritis J. Clin. Invest. 2019-06-06 [PMID: 31169521] (IHC-P, Mouse)	IHC-P	Mouse
Hathaway-Schrader JD, Steinkamp HM, Chavez MB et al. Antibiotic Perturbation of Gut Microbiota Dysregulates Osteoimmune Cross Talk in Postpubertal Skeletal Development. Am. J. Pathol. 2019-02-01 [PMID: 30660331] (FLOW, Mouse)	FLOW	Mouse
Kishimoto A, Kimura S, Nio-Kobayashi J et al. Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy. Exp. Eye Res. 2018-03-27 [PMID: 29596849] (Mouse)		Mouse
Benedicto A, Romayor I, Arteta B. CXCR4 receptor blockage reduces the contribution of tumor and stromal cells to the metastatic growth in the liver. Oncol Rep. 2018-02-08 [PMID: 29436696] (Mouse) Details: This citation used the Biotin form of this antbody		Mouse
Murase T, Yamamoto T, Koide A et al. Temporal expression of chitinase-like 3 in wounded murine skin Int. J. Legal Med. 2017-08-06 [PMID: 28780759] (IF/IHC, Mouse)	IF/IHC	Mouse
Stremnitzer C, Manzano-Szalai K et al. Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation. J Invest Dermatol 2015-01-07 [PMID: 25705851] (IF/IHC, Mouse)	IF/IHC	Mouse
Pickens SR, Chamberlain ND, Volin MV et al. Local expression of interleukin-27 ameliorates collagen-induced arthritis Arthritis Rheum 2011-08-01 [PMID: 21384333] (IF/IHC, Mouse)	IF/IHC	Mouse
Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res;67(9):4346-52. 2007-05-01 [PMID: 17483348] (In Vivo)	In Vivo
Sasmono RT, Ehrnsperger A, Cronau SL, Ravasi T, Kandane R, Hickey MJ, Cook AD, Himes SR, Hamilton JA, Hume DA. Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J Leukoc Biol;82(1):111-23. 2007-07-01 [PMID: 17438263]
Fan J, Li Y, Vodovotz Y, Billiar TR, Wilson MA. Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation. Am J Physiol Lung Cell Mol Physiol;290(4):L738-L746. 2006-04-01 [PMID: 16272176] (In Vivo)	In Vivo
Alvarez MJ, Prada F, Salvatierra E, Bravo AI, Lutzky VP, Carbone C, Pitossi FJ, Chuluyan HE, Podhajcer OL. Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity. Cancer Res;65(12):5123-32. 2005-06-15 [PMID: 15958556]
Brown CR, Blaho VA, Loiacono CM. Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol;171(2):893-901. 2003-07-15 [PMID: 12847259] (IHC-P)	IHC-P
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. 2002-03-15 [PMID: 11877283] (FLOW, Mouse) Details: This citation used the FITC version of this antibody.	FLOW	Mouse
Miura M, Fu X, Zhang QW, Remick DG, Fairchild RL. Neutralization of Gro alpha and macrophage inflammatory protein-2 attenuates renal ischemia/reperfusion injury. Am J Pathol;159(6):2137-45. 2001-12-01 [PMID: 11733364] (IHC-Fr)	IHC-Fr
Dunussi-Joannopoulos K, Runyon K, Erickson J, Schaub RG, Hawley RG, Leonard JP. Vaccines with interleukin-12-transduced acute myeloid leukemia cells elicit very potent therapeutic and long-lasting protective immunity. Blood;94(12):4263-73. 1999-12-15 [PMID: 10590071] (IHC-Fr)	IHC-Fr
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocytedifferentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol 1993-09-01 [PMID: 8360469]
Show All 25 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	02/09/2021	View Summary Application Immunohistochemistry-Paraffin Sample Tested Fetal membranes Species Mouse Lot A-6
Enlarge	4 reviewed by: Verified Customer	IHC-P	Mouse	02/24/2017	View Summary Application Immunohistochemistry-Paraffin Sample Tested mouse skin Species Mouse Comments Comments dilution 1:100 Overnight incubation

Array NBP2-00441

• Ly-6G/Ly-6C Antibodies