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CD11c Antibody (AP-MAB0806) - Azide and BSA Free

 (13 Publications)
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Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Mouse spleen tissue.
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Mouse spleen tissue.
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Negative control, mouse spleen tissue section.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
AP-MAB0806
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-97871AF350
Alexa Fluor 532
NB110-97871AF532
Alexa Fluor 594
NB110-97871AF594
Alexa Fluor 750
NB110-97871AF750
Allophycocyanin/Cy7
NB110-97871APCCY7
DyLight 550
NB110-97871R
DyLight 594
NB110-97871DL594
Janelia Fluor 549
NB110-97871JF549
Janelia Fluor 646
NB110-97871JF646
PE/Atto594
NB110-97871PEATT594
PE/Cy5.5
NB110-97871PECY55
PE/Cy7
NB110-97871PECY7
mFluor Violet 450 SE
NB110-97871MFV450
mFluor Violet 500 SE
NB110-97871MFV500
mFluor Violet 610 SE
NB110-97871MFV610
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CD11c Antibody (AP-MAB0806) - Azide and BSA Free Summary

Immunogen
This CD11c antibody was produced from a hybridoma (mouse myeloma fused with spleen cells)from an Armenian Hamster immunized with mouse lymphocytes.
Marker
Dendritic Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
ITGAX
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100-1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:25-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:25-1:100
  • Immunoprecipitation 1:100
Application Notes
4% PFA fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse CD11c. The antigen retrieval recommended for this antibody is the treatment with 20ug/ml Proteinase K in PBS for 15-25 minutes at room temperature. This antibody is CyTOF ready. Use in ICC/IF reported in scientific literature (PMID: 30300579). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31138656).
Publications
Read Publications using
NB110-97871 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30455684).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration 0.5 mg/ml. Reconstituted antibody can be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity.

Alternate Names for CD11c Antibody (AP-MAB0806) - Azide and BSA Free

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

CD11c (integrin alpha X chain protein) combines with the beta 2 chain (ITGB2) to form a leukocyte specific integrin referred to as inactivated C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. The CD11c antigen is a marker for hairy cell leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (NB110-97871)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(3)
IF/IHC
(7)
IHC-Fr
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB110-97871 Applications Species
Moser V, Workman M, Hurwitz S Et al. Microglial transcription profiles in mouse and human are driven by APOE4 and sex iScience 2021-11-01 [PMID: 34746703] (IF/IHC, Mouse) IF/IHC Mouse
Kim J, Jeong Jh, Jung J et Al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis Rheumatology (Oxford) Mar 12 2020 [PMID: 32163584] (IHC-P, Human) IHC-P Human
Wang G, Xu J, Zhao J et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice Nat Commun 2020-01-10 [PMID: 31924786] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse
Garber C, Soung A et al. T cells promote microglia-mediated synaptic elimination and cognitive dysfunction during recovery from neuropathogenic flaviviruses. Nat Neurosci 2019-06-24 [PMID: 31235930] (IF/IHC, Mouse) IF/IHC Mouse
Butti E, Bacigaluppi M, Chaabane L et al. Neural Stem Cells of the Subventricular Zone Contribute to Neuroprotection of the Corpus Callosum after Cuprizone-Induced Demyelination J. Neurosci. 2019-07-10 [PMID: 31138656] (IHC-Fr, Mouse) IHC-Fr Mouse
Lackington WA, Koci Z, Alekseeva T et al. Genetically induced brain inflammation by Cnp deletion transiently benefits from microglia depletion FASEB J. 2019-05-15 [PMID: 31090455]
Jang Yong Ju, Lim Ji Youn, Kim Seoyeon et al. Enhanced Interferon-beta Response Contributes to Eosinophilic Chronic Rhinosinusitis. Frontiers in Immunology 2018-10-16 [PMID: 30455684] (IF/IHC, Human) IF/IHC Human
Medler T, Murugan D, Horton W et al. Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy Cancer Cell. 2018-10-08 [PMID: 30300579] (ICC/IF, ICC/IF, Mouse) ICC/IF, ICC/IF Mouse
Keren-Shaul H, Spinrad A, Weiner A et al. A Unique Microglia Type Associated with Restricting Development of Alzheimer's Disease. Cell. 2017-06-15 [PMID: 28602351]
Sagar D, Singh NP, Ginwala R et al. Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity. Sci Rep. 2017-06-02 [PMID: 28578388] (IF/IHC, Mouse) IF/IHC Mouse
Show All 13 Publications.

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Product General Protocols

View specific protocols for CD11c Antibody (NB110-97871): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD11c Antibody (NB110-97871). (Showing 1 - 1 of 1 FAQs).

  1. For the following product, please let us know the lyophilized buffer composition. Is it only PBS?
    • This antibody (NB110-97871) is lyophilized and the lyophilized product only contains the antibody and it has to be reconstituted in PBS.

Secondary Antibodies

 

Isotype Controls

Additional CD11c Products

Array NB110-97871

Research Areas for CD11c Antibody (NB110-97871)

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Blogs on CD11c.

Check out the latest blog posts on CD11c.

  Read full blog post.
CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.
Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.
CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol ITGAX
Entrez
Uniprot