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Ly-6G/Ly-6C Antibody (1A8) [Unconjugated]

 (14 Publications)
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Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins.
Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
1A8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB10371U-100UG
Alexa Fluor 405
FAB10371V-100UG
Alexa Fluor 488
FAB10371G-100UG
Alexa Fluor 532
FAB10371X
Alexa Fluor 594
FAB10371T-100UG
Alexa Fluor 647
FAB10371R-100UG
Alexa Fluor 700
FAB10371N-100UG
Alexa Fluor 750
FAB10371S-100UG
Allophycocyanin
FAB10371A
Allophycocyanin/Cy7
FAB10371APCCY7
Biotin
FAB10371B
DyLight 350
FAB10371D
DyLight 405
FAB10371E
DyLight 488
FAB10371K
DyLight 550
FAB10371L
DyLight 594
FAB10371M
DyLight 650
FAB10371W
DyLight 680
FAB10371Y
DyLight 755
FAB10371Z
FITC
FAB10371F
HRP
FAB10371H
Janelia Fluor 549
FAB10371I
Janelia Fluor 646
FAB10371J
PE
FAB10371P
PE/Atto594
FAB10371PEATT594
PE/Cy5.5
FAB10371PECY55
PE/Cy7
FAB10371PECY7
PerCP
FAB10371C
mFluor Violet 450 SE
FAB10371MFV450
mFluor Violet 500 SE
FAB10371MFV500
mFluor Violet 610 SE
FAB10371MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB10371-SP
Carrier Free
FAB10371V-100UG
Carrier Free
FAB10371U-100UG
Carrier Free
FAB10371T-100UG
Carrier Free
FAB10371S-100UG
Carrier Free
FAB10371R-100UG
Carrier Free
FAB10371N-100UG
Carrier Free
FAB10371G-100UG
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Ly-6G/Ly-6C Antibody (1A8) [Unconjugated] Summary

Immunogen
Ly-6G transfected EL-4J cell line
Accession # P35461
Specificity
Detects mouse Ly-6G in Flow Cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB10371 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ly-6G/Ly-6C Antibody (1A8) [Unconjugated]

  • GR
  • Gr1
  • Gr-1
  • Ly-6
  • Ly6G
  • Ly-6G

Background

The myeloid differentiation antigen Gr-1, also known as Ly-6G, is a member of the Ly-6 family (1). The 1A8 antibody binds to Ly-6G while the RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). Ly-6G is a 21-25 kDa glycosyolphosphatidylinositol-anchored protein that is predominatly expressed in granulocytes in bone marrow.
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB10371
Ly-6G/Ly-6C Antibody (1A8) [Unconjugated]
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Publications for Ly-6G/Ly-6C Antibody (MAB10371)(14)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, In Vivo, Neutralization, Neutrophil Depletion.


Filter By Application
Flow Cytometry
(3)
IHC
(4)
In Vivo
(1)
Neutralization
(8)
Neutrophil Depletion
(1)
All Applications
Filter By Species
Mouse
(14)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB10371 Applications Species
Greenman, R;Snir, T;Katav, A;Aricha, R;Mishalian, I;Hay, O;Frankel, M;Lawler, J;Saffioti, F;Pinzani, M;Thorburn, D;Peled, A;Mor, A;Vaknin, I; The Role of CCL24 in Primary Sclerosing Cholangitis: Bridging Patient Serum Proteomics to Preclinical Data Cells 2024-01-23 [PMID: 38334601] (IHC, Mouse) IHC Mouse
JH Riedel, L Robben, HJ Paust, Y Zhao, N Asada, N Song, A Peters, A Kaffke, AC Borchers, G Tiegs, L Seifert, NM Tomas, E Hoxha, UO Wenzel, TB Huber, T Wiech, JE Turner, CF Krebs, U Panzer Glucocorticoids target the CXCL9/10-CXCR3 axis and confer protection against immune-mediated kidney injury JCI Insight, 2023-01-10;0(0):. 2023-01-10 [PMID: 36355429] (Flow Cytometry, Mouse) Flow Cytometry Mouse
A Kumar, PK Singh, Z Ahmed, S Singh, A Kumar Essential Role of NLRP3 Inflammasome in Mediating IL-1beta Production and the Pathobiology of Staphylococcus aureus Endophthalmitis Infection and Immunity, 2022-04-11;0(0):e0010322. 2022-04-11 [PMID: 35404106] (Flow Cytometry, In Vivo, Mouse, Transgenic Mouse) Flow Cytometry, In Vivo Mouse, Transgenic Mouse
M Terasawa, K Hiramoto, R Uchida, K Suzuki Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium Marine Drugs, 2022-02-03;20(2):. 2022-02-03 [PMID: 35200650] (IHC, Mouse) IHC Mouse
KHINM Herath, HJ Kim, A Kim, CE Sook, BY Lee, Y Jee The Role of Fucoidans Isolated from the Sporophylls of Undaria pinnatifida against Particulate-Matter-Induced Allergic Airway Inflammation: Evidence of the Attenuation of Oxidative Stress and Inflammatory Responses Molecules, 2020-06-22;25(12):. 2020-06-22 [PMID: 32580518] (IHC, Mouse) IHC Mouse
N Gupta, PK Singh, SG Revankar, PH Chandrasek, A Kumar Pathobiology of Aspergillus Fumigatus Endophthalmitis in Immunocompetent and Immunocompromised Mice Microorganisms, 2019-08-28;7(9):. 2019-08-28 [PMID: 31466325] (Neutrophil Depletion, Mouse) Neutrophil Depletion Mouse
Kimmey J, Huynh J, Weiss L, Park S, Kambal A, Debnath J, Virgin H, Stallings C Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection. Nature, 2015-12-09;528(7583):565-9. 2015-12-09 [PMID: 26649827] (Neutralization, Mouse) Neutralization Mouse
Yipp B, Kubes P Antibodies against neutrophil LY6G do not inhibit leukocyte recruitment in mice in vivo. Blood, 2013-01-03;121(1):241-2. 2013-01-03 [PMID: 23287627] (Neutralization, Mouse) Neutralization Mouse
Jarchum I, Liu M, Shi C, Equinda M, Pamer E Critical role for MyD88-mediated neutrophil recruitment during Clostridium difficile colitis. Infect Immun, 2012-06-11;80(9):2989-96. 2012-06-11 [PMID: 22689818] (IHC, Neutralization, Mouse) IHC, Neutralization Mouse
Tate M, Ioannidis L, Croker B, Brown L, Brooks A, Reading P The role of neutrophils during mild and severe influenza virus infections of mice. PLoS ONE, 2011-03-14;6(3):e17618. 2011-03-14 [PMID: 21423798] (Neutralization, Mouse) Neutralization Mouse
Show All 14 Publications.

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