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KIR2DL3/CD158b2 Antibody (180701) [Unconjugated]

 (11 Publications)
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Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P).View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
180701
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2014U-100UG
Alexa Fluor 405
FAB2014V-100UG
Alexa Fluor 488
FAB2014G-100UG
Alexa Fluor 532
FAB2014X
Alexa Fluor 594
FAB2014T-100UG
Alexa Fluor 647
FAB2014R-100UG
Alexa Fluor 700
FAB2014N-100UG
Alexa Fluor 750
FAB2014S-100UG
Allophycocyanin
FAB2014A-025
Allophycocyanin
FAB2014A-100
Allophycocyanin/Cy7
FAB2014APCCY7
Biotin
FAB2014B
DyLight 350
FAB2014D
DyLight 405
FAB2014E
DyLight 488
FAB2014K
DyLight 550
FAB2014L
DyLight 594
FAB2014M
DyLight 650
FAB2014W
DyLight 680
FAB2014Y
DyLight 755
FAB2014Z
Fluorescein
FAB2014F-025
Fluorescein
FAB2014F-100
HRP
FAB2014H
Janelia Fluor 549
FAB2014I
Janelia Fluor 646
FAB2014J
PE/Atto594
FAB2014PEATT594
PE/Cy5.5
FAB2014PECY55
PE/Cy7
FAB2014PECY7
Phycoerythrin
FAB2014P-100
Phycoerythrin
FAB2014P-025
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB2014F-025
Carrier Free
FAB2014F-100
Carrier Free
FAB2014P-025
Carrier Free
FAB2014P-100
Carrier Free
FAB2014A-025
Carrier Free
FAB2014A-100
Carrier Free
MAB2014-SP
Carrier Free
FAB2014V-100UG
Carrier Free
FAB2014U-100UG
Carrier Free
FAB2014T-100UG
Carrier Free
FAB2014S-100UG
Carrier Free
FAB2014R-100UG
Carrier Free
FAB2014N-100UG
Carrier Free
FAB2014G-100UG
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KIR2DL3/CD158b2 Antibody (180701) [Unconjugated] Summary

Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
Accession # P43628
Specificity
Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
KIR2DL3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB2014 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL3/CD158b2 Antibody (180701) [Unconjugated]

  • CD158 antigen-like family member B2
  • CD158b2 antigen
  • CD158b2
  • CD158B2CD158b
  • cl-6
  • killer cell immunoglobulin-like receptor 2DL3
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
  • Killer inhibitory receptor cl 2-3
  • KIR-023GB
  • KIR2DL3
  • KIRCL23
  • KIR-K7b
  • KIR-K7c
  • MGC129943
  • MHC class I NK cell receptor
  • natural killer associated transcript 2
  • natural killer cell inhibitory receptor KIR2DL3
  • Natural killer-associated transcript 2
  • NKAT
  • NKAT2
  • NKAT-2
  • NKAT2A
  • nkat2b
  • NKAT2GL183
  • NK-receptor
  • p58 natural killer cell receptor clone CL-6
  • p58 NK receptor CL-6
  • p58
  • p58.2 MHC class-I specific NK receptor
  • p58.2 MHC class-I-specific NK receptor

Background

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KIR2DL3/CD158b2 Antibody (MAB2014)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: CyTof, Flow Cytometry, Flow Cytometry Control.


Filter By Application
CyTof
(1)
Flow Cytometry
(9)
Flow Cytometry Control
(1)
All Applications
Filter By Species
Human
(11)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB2014 Applications Species
L Dubreuil, B Maniangou, P Chevallier, A Quéméner, N Legrand, MC Béné, C Willem, G David, M Alizadeh, DR Makanga, A Cesbron, K Gendzekhad, K Gagne, C Retière Centromeric KIR AA Individuals Harbor Particular KIR Alleles Conferring Beneficial NK Cell Features with Implications in Haplo-Identical Hematopoietic Stem Cell Transplantation Cancers, 2020-12-01;12(12):. 2020-12-01 [PMID: 33271841] (Flow Cytometry, Human) Flow Cytometry Human
L Gimeno, EM Serrano-Ló, JA Campillo, MA Cánovas-Za, OS Acuña, F García-Cóz, MV Martínez-S, MD Martínez-H, MF Soto-Ramír, P López-Cubi, J Martínez-E, J Martínez-G, S Corbalan-G, MR Álvarez-Ló, A Minguela KIR+ CD8+ T Lymphocytes in Cancer Immunosurveillance and Patient Survival: Gene Expression Profiling Cancers (Basel), 2020-10-15;12(10):. 2020-10-15 [PMID: 33076479] (Flow Cytometry, Human) Flow Cytometry Human
A Koenig, CC Chen, A Marçais, T Barba, V Mathias, A Sicard, M Rabeyrin, M Racapé, JP Duong-Van-, P Bruneval, A Loupy, S Dussurgey, S Ducreux, V Meas-Yedid, JC Olivo-Mari, H Paidassi, R Guillemain, JL Taupin, J Callemeyn, E Morelon, A Nicoletti, B Charreau, V Dubois, M Naesens, T Walzer, T Defrance, O Thaunat Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants Nat Commun, 2019-11-25;10(1):5350. 2019-11-25 [PMID: 31767837] (Flow Cytometry, Human) Flow Cytometry Human
HIV-1 Control by NK Cells via Reduced Interaction between KIR2DL2 and HLA-C(?)12:02/C(?)14:03 Cell Rep, 2016-11-22;17(9):2210-2220. 2016-11-22 [PMID: 27880898] (Flow Cytometry, Human) Flow Cytometry Human
NK Cell Responses to Human Tick-Borne Encephalitis Virus Infection J Immunol, 2016-08-19;0(0):. 2016-08-19 [PMID: 27543616] (Flow Cytometry, Human) Flow Cytometry Human
Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. J Immunol, 2015-09-28;195(9):4524-36. 2015-09-28 [PMID: 26416275] (CyTof, Human) CyTof Human
Sharkey A, Xiong S, Kennedy P, Gardner L, Farrell L, Chazara O, Ivarsson M, Hiby S, Colucci F, Moffett A Tissue-Specific Education of Decidual NK Cells. J Immunol, 2015-08-28;195(7):3026-32. 2015-08-28 [PMID: 26320253] (Flow Cytometry, Human) Flow Cytometry Human
Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Beziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Onfelt B, Malmberg K Coordinated expression of DNAM-1 and LFA-1 in educated NK cells. J Immunol, 2015-03-30;194(9):4518-27. 2015-03-30 [PMID: 25825444] (Flow Cytometry, Human) Flow Cytometry Human
Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT. Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96. 2014-12-15 [PMID: 25512551] (Flow Cytometry Control, Human) Flow Cytometry Control Human
Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection. Eur J Immunol, 2012-12-12;43(2):480-7. 2012-12-12 [PMID: 23161492] (Flow Cytometry, Human) Flow Cytometry Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol KIR2DL3
Uniprot