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UBASH3B/STS1/Tula-2 Antibody

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Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U-118-MG human glioblastoma/astrocytoma cell line, U2OS human osteosarcoma cell line, and Y3-Ag rat myeloid cell line. PVDF Membrane was probed with 2 µg/mL of Human/Rat UBASH3B/STS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6696) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBASH3B/STS1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U-118-MG human glioblastoma/astrocytoma cell ...read more
UBASH3B/STS1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat UBASH3B/STS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6696) at 10 µg/mL ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6696-SP
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UBASH3B/STS1/Tula-2 Antibody Summary

Immunogen
E. coli-derived recombinant human UBASH3B/STS1
Gly48-Phe253
Accession # Q8TF42
Specificity
Detects human and rat UBASH3B/STS1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
UBASH3B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UBASH3B/STS1/Tula-2 Antibody

  • Cbl-interacting Protein p70
  • Cbl-interacting protein Sts-1
  • KIAA1959SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling1, nm23-phosphorylated unknown substrate
  • MGC15437
  • nm23-phosphorylated unknown substrate
  • p70
  • STS1
  • STS1EC 3.1.3.48
  • STS-1ubiquitin-associated and SH3 domain-containing protein B
  • Suppressor of T-cell receptor signaling 1
  • T-cell ubiquitin ligand 2
  • TULA-2
  • Tyrosine-protein phosphatase STS1/TULA2
  • UBASH3B
  • ubiquitin associated and SH3 domain containing B

Background

UBASH3B (Ubiquitin-associated domain SH3 domain-containing protein B; also STS1 and TULA2) is a cytosolic 72-74 kDa member of the TULA (T cell ubiquitin ligand) family of proteins. It is widely expressed and appears to perform multiple functions. As a histidine phosphatase, it dephosphorylates Syk and Syk-related molecules, thus dampening signaling pathways. Via ubiquitin and SH3 binding domains, it binds to monoubiquitinated RTKs and Cbl, thereby increasing RTK activity by blocking its polyubiquitination and subsequent lysosomal degradation. There is also the potential for phosphodiesterase activity directed towards tRNA activity. Human UBASH3B is 649 amino acids (aa) in length. It contains one UBA domain (aa 39-75), a proline-rich ligand binding SH3 region (aa 257-316), and a PGM/histidine phosphatase domain (aa 386-598). UBASH3B is suggested to dimerize. Over aa 47-253, human and mouse UBASH3B are identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBASH3B
Uniprot