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JWA Antibody

Images

 
Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Small Intestine with antibody at 3.75 g/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: JWA Antibody [NBP3-12158] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Tonsil with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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JWA Antibody Summary

Immunogen
Peptide with sequence C-NRLTDYISKVKE corresponding to C-Terminus according to NP_006398.1.
Clonality
Polyclonal
Host
Goat
Gene
ARL6IP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JWA Antibody

  • ADP-ribosylation factor-like protein 6-interacting protein 5
  • ADP-ribosylation-like factor 6 interacting protein 5
  • Aip-5
  • ARL-6-interacting protein 5
  • Cytoskeleton-related vitamin A-responsive protein
  • Dermal papilla-derived protein 11
  • DERP11addicsin
  • Glutamate transporter EAAC1-interacting protein
  • glutamate transporter EEAC1-associated protein
  • GTRAP3-18aip-5
  • hp22
  • HSPC127
  • JM5
  • jmx
  • JWAcytoskeleton related vitamin A responsive protein
  • PRA1 domain family 3
  • PRA1 family protein 3
  • PRA2
  • PRAF3dermal papilla derived protein 11
  • Prenylated Rab acceptor protein 2
  • Protein JWa
  • putative MAPK activating protein PM27
  • Putative MAPK-activating protein PM27

Background

Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional JWA Products

Array NBP3-12158

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Bioinformatics

Gene Symbol ARL6IP5