HAP1 Antibody (1B6)

HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IP, WB.

Showing Publications 1 - 9 of 9.

Publications using NB110-74569	Applications	Species
Cason SE, Carman PJ, Van Duyne C et al. Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway Journal of Cell Biology 2021-07-05 [PMID: 34014261]
Gong YJ, Feng Y, Cao YY et al. Huntingtin-associated protein 1 plays an essential role in the pathogenesis of type 2 diabetes by regulating the translocation of GLUT4 in mouse adipocytes BMJ Open Diabetes Res Care 2020-10-01 [PMID: 33060070] (IF/IHC, Mouse)	IF/IHC	Mouse
Xiang J, Yang S, Xin N et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome Proc. Natl. Acad. Sci. U.S.A 2017-01-30 [PMID: 28137862] (IP, Mouse)	IP	Mouse
Corradini E, Burgers PP, Plank M et al. Huntingtin associated protein (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP), specific for the cGMP-dependent protein kinase Ibeta isoform J. Biol. Chem. 2015-02-04 [PMID: 25653285] (WB, Human) Details: HAP1 antibody used for WB analysis of totall cell lysates as well as the pulldowns performed in COS-7 cells which were co-transfected with HAP1 alone or with either PKG I alpha or PKG I beta (FIGURE 2). WB also performed on lysates and proteins pulled down in COS-7 cells co-expressing PKG I beta-GFP plasmid with HAP1-DSRed construct (Control), HAP1-DSRed bearing the double mutation K567A/R568A, and C-terminal truncated HAP1-DSRed construct /HAP1 amino acids 157-386 (FIGURE 6).	WB	Human
Corradini E, Vallur R, Raaijmakers LM et al. Alterations in the cerebellar (phospho)proteome of a cGMP-dependent protein kinase knockout mouse. Mol. Cell Proteomics. 2014-06-12 [PMID: 24925903] (WB, Mouse) Details: 6 week-old female WT and cGKI-KO mice (carrying a null mutation of the prkg1 gene, also termed cGKIL-/L- mice ) on a 129/Sv background were sacrificed and cerebella were kept frozen until testing. The tissues were lysed by sonication in lysis buffer and processed for WB using mouse anti-Huntingtin associated protein 1 (HAP1, Novus biological, 1:5000). HAP1 showed an increased expression in the cerebellum of cGKI-KO mice.	WB	Mouse
Tang TS, Tu H, Chan EY, Maximov A, Wang Z, Wellington CL, Hayden MR, Bezprozvanny I. Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1. Neuron;39(2):227-39. 2003-07-17 [PMID: 12873381] (IP, Rat, Mouse)	IP	Rat, Mouse
Torre ER, Coleman S, Yi H et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 2003-05-01 [PMID: 12763227] (WB, Rat)	WB	Rat
Gutekunst, C et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem;51(6):841-52. 2003-06-01 [PMID: 12754295] (ICC/IF, WB, Rat, Mouse)	ICC/IF, WB	Rat, Mouse
Chan, E et al. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet;11(8):945-59. 2002-04-15 [PMID: 11971876] (WB, Mouse)	WB	Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human, Mouse, Rat	06/19/2018	View Summary Application Western Blot Sample Tested Hela whole cell lysate,Mouse brain,PC12 cell lysate Species Human, Mouse, Rat Lot A

Array NB110-74569

• HAP1 Antibodies