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Recombinant Human CYP27B1 GST (N-Term) Protein

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12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, AP

Order Details

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Recombinant Human CYP27B1 GST (N-Term) Protein Summary

Description
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 409-508 of Human CYP27B1

Source: Wheat Germ (in vitro)

Amino Acid Sequence: TLCHYATSRDPAQFPEPNSFRPARWLGEGPTPHPFASLPFGFGKRSCMGRRLAELELQMALAQILTHFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
CYP27B1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
36.74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CYP27B1 GST (N-Term) Protein

  • 1alpha(OH)ase
  • 25 hydroxyvitamin D3-1-alpha hydroxylase
  • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
  • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
  • 25-OHD-1 alpha-hydroxylase
  • Calcidiol 1-monooxygenase
  • CP2B
  • CYP1
  • CYP1ALPHA
  • CYP27B
  • CYP27B1
  • Cytochrome p450 27B1
  • Cytochrome P450 subfamily XXVIIB polypeptide 1
  • cytochrome P450, family 27, subfamily B, polypeptide 1
  • cytochrome P450, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase)
  • Cytochrome P450C1 alpha
  • Cytochrome P450VD1-alpha
  • EC 1.14.13.13
  • EC 1.14.15.18
  • P450c1
  • PDDR
  • polypeptide 1
  • VD3 1A hydroxylase
  • VDD1
  • VDDR
  • VDDRI
  • VDR

Background

CYP27B1 - cytochrome P450, family 27, subfamily B, polypeptide 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CYP27B1