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GAD1/GAD67 Antibody (252910)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
252910
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB2086-SP
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GAD1/GAD67 Antibody (252910) Summary

Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Specificity
Detects human GAD1/GAD67 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GAD1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAD1/GAD67 Antibody (252910)

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

GAD1, also known as GAD67, is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions and putative paracrine hormone in pancreatic islets. GAD occurs as two molecular forms, 65 kDa and 67 kDa, that are encoded by separate genes and share 65% amino acid identity. GAD1 is highly conserved and is expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary (1).
  1. Sheikh, S.N. et al. (1999) Neurochem. Int. 35:73.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GAD1
Uniprot