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FosB/G0S3 Antibody (83B1138) - BSA Free

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Western Blot: FosB/G0S3 Antibody (83B1138) [NB100-56530] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB.
Western Blot: FosB/G0S3 Antibody (83B1138) [NB100-56530] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
83B1138
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56530AF350
Alexa Fluor 405
NB100-56530AF405
Alexa Fluor 488
NB100-56530AF488
Alexa Fluor 532
NB100-56530AF532
Alexa Fluor 594
NB100-56530AF594
Alexa Fluor 647
NB100-56530AF647
Alexa Fluor 700
NB100-56530AF700
Alexa Fluor 750
NB100-56530AF750
Biotin
NB100-56530B
CoraFluor 1
NB100-56530CL1
DyLight 350
NB100-56530UV
DyLight 405
NB100-56530V
DyLight 488
NB100-56530G
DyLight 550
NB100-56530R
DyLight 594
NB100-56530DL594
DyLight 650
NB100-56530C
DyLight 680
NB100-56530FR
DyLight 755
NB100-56530IR
FITC
NB100-56530F
HRP
NB100-56530H
Janelia Fluor 525
NB100-56530JF525
Janelia Fluor 549
NB100-56530JF549
Janelia Fluor 585
NB100-56530JF585
Janelia Fluor 635
NB100-56530JF635
Janelia Fluor 646
NB100-56530JF646
Janelia Fluor 669
NB100-56530JF669
mFluor Violet 450 SE
NB100-56530MFV450
mFluor Violet 500 SE
NB100-56530MFV500
mFluor Violet 610 SE
NB100-56530MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB100-56530SS
Azide Free
NBP2-33219
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FosB/G0S3 Antibody (83B1138) - BSA Free Summary

Immunogen
This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 2-15 of human FosB.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOSB
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Application Notes
In WB of HeLa cells, a 46 kDa band is observed.
Control
HeLa Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FosB/G0S3 Antibody (83B1138) - BSA Free

  • activator protein 1
  • AP-1
  • DKFZp686C0818
  • FBJ murine osteosarcoma viral oncogene homolog B
  • FosB
  • G0S3
  • G0S3G0/G1 switch regulatory protein 3
  • GOS3
  • GOSB
  • MGC42291
  • oncogene FOS-B
  • protein fosB

Background

The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a 62 kDa nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo and heterodimers, which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family. Murine FosB encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B which bind to TRE DNA response elements. Functional homologs of c-Fos and FosB include Fra-1 and Fra-2 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FosB/G0S3 Antibody (NB100-56530) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FosB/G0S3 Antibody (NB100-56530). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in your product, NB100-56530 FosB/G0S3 Antibody, and would like know if this item cross react to mouse. Have you done any in-house test for mouse especially on brain tissue sample. If yes, could you kindly provide some testing result?
    • This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 2-15 of human FosB. This immunogen sequence is 100% analogous to the mouse variant (https://www.uniprot.org/uniprot/P13346), so NB100-56530 will very likely cross react to the mouse protein. Because we have only validated this AB for use in human, we have not done any in-house tests for mouse and don't have any material to offer you. We cannot guarantee that this AB will work for mouse FosB/G0S3, but there is a strong likelihood that it will. Please note that this antibody is hosted in mouse though, which could potentially affect your results since you will need to use an anti-mouse secondary (which in theory will react with the endogenous mouse IgGs in the sample itself). However, since we have not tested this explicitly in mouse, we do not know what the results would look like.

Control Lysate(s)

HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FOSB
Entrez
Uniprot