![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0009.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | IHC, MA |
| Clone | ENO2/1462 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | Human Chromosome Location:12p13 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59603) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104) |
| Localization | Cytoplasmic |
| Marker | Neuroendocrine Marker |
| Specificity | The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENO2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Enolase 2/Neuron-specific Enolase Antibody (NBP2-59602)Find related products by research area.
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![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained withNSE gamma Monoclonal Antibody (ENO2/1462).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0007.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained withNSE gamma Monoclonal Antibody (ENO2/1462).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained withNSE gamma Monoclonal Antibody (ENO2/1462)](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0008.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained withNSE gamma Monoclonal Antibody (ENO2/1462)")
![Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Protein-Array-NBP2-59602-img0012.jpg "Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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