![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Analysis of Y79, HeLa and HepG2 Cell Lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Western-Blot-NBP2-47635-img0003.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Analysis of Y79, HeLa and HepG2 Cell Lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | ENO2/1375 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54452) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104) |
| Localization | Cytoplasmic |
| Marker | Neuroendocrine Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENO2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.2-0.4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Enolase 2/Neuron-specific Enolase Antibody (NBP2-47635)Find related products by research area.
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![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffinembedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0006.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffinembedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
![Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - An intracellular stain was performed on HeLa cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image from the Allophycocyanin version of this antibody.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Flow-Intracellular-NBP2-47635-img0008.jpg "Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - An intracellular stain was performed on HeLa cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image from the Allophycocyanin version of this antibody.")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0001.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffin-embedded Human cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0004.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffin-embedded Human cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffin-embedded mouse pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0005.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffin-embedded mouse pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
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