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Doublecortin Antibody (2G5) - BSA Free

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Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mouse mAb against Mouse heart (1) lysate.
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (2G5) [NBP2-37476] - Immunofluorescence analysis of HepG2 cells using DCX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of kidney cancer tissues using DCX mouse mAb with DAB staining.
Flow Cytometry: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of SK-N-SH cells using DCX mouse mAb (green) and negative control (red).
Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mAb against human DCX recombinant protein. (Expected MW is 34.1 kDa)
Immunohistochemistry-Paraffin: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of brain tissue tissues using DCX mouse mAb with DAB staining.
ELISA: Doublecortin Antibody (2G5) [NBP2-37476] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
2G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Doublecortin Antibody (2G5) - BSA Free Summary

Description
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Immunogen
Purified recombinant fragment of human Doublecortin (AA: 362-411) expressed in E. Coli.
Localization
Cell projection, Cytoplasm, Microtubule
Specificity
Doublecortin's Isoform a (NP_000546.2) and other isoforms.
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DCX
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
Doublecortin's Isoform a (NP_000546.2) is larger than other isoforms and this antibody may detect other isoforms of this protein.
Theoretical MW
49.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human and Mouse. Predicted to react with Rat (91%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Doublecortin Antibody (2G5) - BSA Free

  • DBCN
  • Doublecortex
  • Doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • LISX
  • Lis-X
  • X-linked (doublecortin)

Background

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DCX