Doublecortin Antibody (2G5) - BSA Free Summary
Description |
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. |
Immunogen |
Purified recombinant fragment of human Doublecortin (AA: 362-411) expressed in E. Coli. |
Localization |
Cell projection, Cytoplasm, Microtubule |
Specificity |
Doublecortin's Isoform a (NP_000546.2) and other isoforms. |
Predicted Species |
Rat (91%). Backed by our 100% Guarantee. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
DCX |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:10000
- Flow Cytometry 1:200 - 1:400
- Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
- Immunohistochemistry 1:200 - 1:1000
- Immunohistochemistry-Paraffin 1:200 - 1:1000
- Western Blot 1:500 - 1:2000
|
Application Notes |
Doublecortin's Isoform a (NP_000546.2) is larger than other isoforms and this antibody may detect other isoforms of this protein. |
Theoretical MW |
49.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Reacts with Human and Mouse. Predicted to react with Rat (91%).
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for Doublecortin Antibody (2G5) - BSA Free
Background
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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