![Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DBF4-Antibody-6G9-Western-Blot-H00010926-M01-img0006.jpg "Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 6G9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | DBF4 (NP_006707, 2 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQT |
| Specificity | DBF4 - DBF4 homolog (S. cerevisiae) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DBF4 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00010926-M01 | Applications | Species |
|---|---|---|
| Hughes S, Jenkins V, Dar MJ et al. Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem 2010-01-01 [PMID: 19864417] |
Secondary Antibodies |
Isotype Controls |
Research Areas for DBF4 Antibody (H00010926-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/DBF4-Antibody-6G9-Immunocytochemistry-Immunofluorescence-H00010926-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]](http://images.novusbio.com/fullsize/DBF4-Antibody-6G9-Immunohistochemistry-Paraffin-H00010926-M01-img0001.jpg "Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]")
![Sandwich ELISA: DBF4 Antibody (6G9) [H00010926-M01] - Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DBF4-Antibody-6G9-Sandwich-ELISA-H00010926-M01-img0005.jpg "Sandwich ELISA: DBF4 Antibody (6G9) [H00010926-M01] - Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")