![Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80627-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody. Image from the standard format of this antibody.")
| Reactivity | Hu, Po, Bv, Ca, PmSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, B/N, CyTOF-ready |
| Clone | M5E2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Clone M5E2 was used by HLDA to establish CD designation. |
| Immunogen | Human peripheral blood cells. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD14 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CD14 |
![Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80627-img0006.jpg "Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80627-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.")
![Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80627-img0003.jpg "Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image from the standard format of this antibody.")
![Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were directly conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80627-img0004.jpg "Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were directly conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.")
![Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80627-img0005.jpg "Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.")
![Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human peripheral blood monocytes stained with M5E2 APC Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80627-img0007.jpg "Flow Cytometry: CD14 Antibody (M5E2) - Azide and BSA Free [NBP2-80627] - Human peripheral blood monocytes stained with M5E2 APC Image from the standard format of this antibody.")
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