CD14 Antibodies
We offer CD14 Antibodies for use in common research applications: Blocking/Neutralizing, ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow (Cell Surface), Flow Cytometry, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Peptide ELISA, Sandwich ELISA, Western Blot. Each CD14 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD14 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Feline, Goat, Human, Llama, Mink, Monkey, Mouse, Mustelid, Porcine, Primate, Rabbit, Sheep. Use the list below to choose the CD14 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD14 polyclonal antibodies and browse our CD14 monoclonal antibody catalog.
Alternate Names for CD14 Antibodies
anti-CD14 antibody, anti-CD14 antigen antibody, anti-CD14 molecule antibody, anti-monocyte differentiation antigen CD14 antibody, anti-Myeloid cell-specific leucine-rich glycoprotein antibody
37 Results for "cd14" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Immunocytochemistry-Immunofluorescence-NB100-77758-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody.")
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Immunocytochemistry-Immunofluorescence-NB100-77758-img0008.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Human peripheral blood monocytes stained with M5E2 APC](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0001.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0004.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0005.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0006.jpg)
![Dual RNAscope ISH-IHC: CD14 Antibody (M5E2) [NB100-77758] - TNFA mRNA (red) and CD14 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human malignant lymph node. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-TNFA and CD14 antibody (M5E2) at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Dual-RNAscope-ISH-IHC-NB100-77758-img0010.jpg)
![Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Dual-RNAscope-ISH-IHC-NBP2-37291-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Confocal image of hCD14-transfected HEKT cells, 4% formalin fixed, indirectly labeled with anti-CD14 1:200, and AF568-conjugated Ab 1:1000. Note that cells have different immunoreactivity due to various expression level of CD14 in single cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Immunocytochemistry-NBP2-37291-img0007.jpg)
![Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Flow-Cytometry-NBP2-37291-img0008.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0002.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - BMDM were unchallenged (control) or challenged with 500 HU/mL SLO WT, PFO or an equivalent mass of SLO ML for 10 min at 37C and centrifuged at 2000 x g for 5 min to yield cell pellet (C). Supernatants were spun at 100,000xg for 40 min at 4C to collect the high speed supernatant (S) and microvesicle pellet (M). Samples were solubilized at 95C in SDS-sample buffer, resolved by SDS-PAGE and transferred to nitrocellulose. Portions of the blot were probed with 6D11 anti-SLO, anti-IFNgammaR1, 4B4F12 anti-CD14, 76B357.1 anti-TLR4, O91B8 anti-MyD88, 1H4B01 anti-Trif, EPR4477 anti-Alkaline Phosphatase, MANLAC-4A7 anti-Lamin A/C, and AC-15 anti-beta-Actin, followed by relevant secondary antibodies and ECL. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24955-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0010.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0003.jpg)
![ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-ELISA-NBP2-37291-img0001.jpg)
![Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis of CD14 in human PBMCs using anti-CD14 DyLight 405 conjugated antibody. Image from verified customer review. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-RPA-M1-Flow-Cytometry-NBP2-25194-img0010.jpg "Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis of CD14 in human PBMCs using anti-CD14 DyLight 405 conjugated antibody. Image from verified customer review. Image using the Azide Free format of this antibody.")
![Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Cell surface analysis of CD14 in 10^6 human monocytes using 0.1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD14 antibody. cell surface flow kit and anti-mouse IgG-FITC secondary was used for this test.](http://images.novusbio.com/fullsize/CD14-Antibody-RPA-M1-Flow-Cytometry-NBP2-25194-img0001.jpg)
![Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Cell surface analysis of CD14 PerCP/Cy5.5 in 10^6 human monocytes using 10 ul of this antibody. Green represents PerCP-Cy5.5 conjugated isotype control; red represents anti-CD14 antibody. Cell surface flow kit was used for this test.](http://images.novusbio.com/fullsize/CD14-Antibody-RPA-M1-Flow-Cytometry-NBP2-25194-img0004.jpg)
![Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis using the Azide Free version of NBP2-25194. Staining of CD14 in human PBMCs using anti-CD14 DyLight 405 conjugated antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-RPA-M1-Flow-Cytometry-NBP2-25194-img0006.jpg)
![Flow (Cell Surface): CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis using the FITC conjugate of NBP2-25194. Staining of CD14 in 10^6 human monocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD14 antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-RPA-M1-Flow-Cell-Surface-NBP2-25194-img0007.jpg)
![Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-25194. Staining of CD14 using CD14 monoclonal antibody. Surface staining of CD14 on 10^6 human monocytes using 10 ul of CD14 antibody. Green represents isotype control antibody; red represents CD14](http://images.novusbio.com/fullsize/CD14-Antibody-RPA-M1-Flow-Cytometry-NBP2-25194-img0008.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86168 (A) shows similar protein distribution across tissues to independent antibody NBP1-86166 (B).](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CD14 Antibody [NBP1-86168] - Analysis using Anti-CD14 antibody NBP1-86168 (A) shows similar pattern to independent antibody NBP1-86166 (B).](http://images.novusbio.com/fullsize/CD14-Antibody-Western-Blot-NBP1-86168-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody [NBP1-86168] - Staining of human cell line SiHa shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86168-img0007.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0021.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0017.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0018.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0019.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0020.jpg)
![Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Western-Blot-NBP2-37295-img0003.jpg "Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of HepG2 cells using CD14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunocytochemistry-NBP2-37295-img0008.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (1H5D8) [NBP2-37295] - Human tonsil FFPE tissue. Antigen retrieval with EDTA buffer. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunohistochemistry-Paraffin-NBP2-37295-img0009.jpg)
![Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Western-Blot-NBP2-37295-img0002.jpg)
![Immunohistochemistry: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of paraffin-embedded cervical cancer tissues using CD14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunohistochemistry-NBP2-37295-img0005.jpg)
![Immunohistochemistry: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of paraffin-embedded colon tissues using CD14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunohistochemistry-NBP2-37295-img0007.jpg)
![ELISA: CD14 Antibody (1H5D8) [NBP2-37295] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-ELISA-NBP2-37295-img0001.jpg)
![Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of CD14 on NIH/3T3 cell lysates with Rabbit anti-CD14 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Western-Blot-NBP2-67630-img0012.jpg "Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of CD14 on NIH/3T3 cell lysates with Rabbit anti-CD14 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0010.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0009.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0003.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0004.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0005.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0006.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0007.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0013.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0014.jpg)
![Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - analysis of CD14 on different lysates with Rabbit anti-CD14 antibody at 1/1,000 dilution.Lane 1: SW480-si NT cell lysateLane 2: SW480-si CD14 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 40 kDaObserved band size: 50-60 kDaExposure time: 7 seconds;ECL: merk4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-67630_rabbit-monoclonal-cd14-antibody-sc69-02-western-blot-264202493347..jpg)
![Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - analysis of CD14 on different lysates with Rabbit anti-CD14 antibody at 1/5,000 dilution.Lane 1: THP-1 cell lysateLane 2: RAW264.7 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 40 kDaObserved band size: 60 kDaExposure time: 1 minute 40 seconds;4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-67630_rabbit-monoclonal-cd14-antibody-sc69-02-western-blot-264202493452..jpg)
![Western Blot: CD14 Antibody [NBP1-86166] - Analysis using Anti-CD14 antibody NBP1-86166 (A) shows similar pattern to independent antibody NBP1-86168 (B).](http://images.novusbio.com/fullsize/CD14-Antibody-Western-Blot-NBP1-86166-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CD14 Antibody [NBP1-86166] - Analysis using Anti-CD14 antibody NBP1-86166 (A) shows similar pattern to independent antibody NBP1-86168 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86166 (A) shows similar protein distribution across tissues to independent antibody NBP1-86168 (B).](http://images.novusbio.com/fullsize/CD14-Antibody-Western-Blot-NBP1-86166-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0014.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0015.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0016.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0017.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0019.jpg)
![Western Blot: CD14 Antibody [NB100-2807] - Staining (1ug/ml) of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD14-Antibody-Flow-Cytometry-NB100-2807-img0006.jpg "Western Blot: CD14 Antibody [NB100-2807] - Staining (1ug/ml) of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.")
![Flow Cytometry: CD14 Antibody [NB100-2807] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-Flow-Cytometry-NB100-2807-img0005.jpg)
![Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] - (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/antibody/nb100-2807_goat-polyclonal-cd14-antibody-imgenex-img-3991-immunohistochemistry-283202413831..jpg)
![Control: Goat Polyclonal CD14 Antibody [NB100-2807] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/antibody/nb100-2807_goat-polyclonal-cd14-antibody-imgenex-img-3991-control-283202413949..jpg)