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AP1M2 Overexpression Lysate

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Western Blot: AP1M2 Overexpression Lysate (Adult Normal) [NBP2-07741] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AP1M2.
Western Blot: AP1M2 Overexpression Lysate (Adult Normal) [NBP2-07741] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AP1M2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

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AP1M2 Overexpression Lysate Summary

Description

AP1M2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202818

Accession#: NM_005498

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
AP1M2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for AP1M2 Overexpression Lysate

  • Adaptor protein complex AP-1 mu-2 subunit
  • Adaptor-related protein complex 1 mu-2 subunit
  • adaptor-related protein complex 1, mu 2 subunit
  • AP-1 complex subunit mu-2
  • AP1-mu2
  • AP-mu chain family member mu1B
  • Clathrin assembly protein complex 1 medium chain 2
  • clathrin coat assembly protein AP47 2
  • clathrin coat associated protein AP47 2
  • clathrin-associated adaptor medium chain mu2
  • golgi adaptor AP-1 47 kDa protein
  • Golgi adaptor HA1/AP1 adaptin mu-2 subunit
  • HA1 47 kDa subunit 2
  • HSMU1B
  • MU1B
  • MU-1B
  • mu1B-adaptin
  • mu2
  • Mu-adaptin 2

Background

AP1M2 encodes a subunit of the heterotetrameric adaptor-related protein comlex 1 (AP-1), which belongs to the adaptor complexes medium subunits family. This protein is capable of interacting with tyrosine-based sorting signals. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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AP1M2
Overexpression Lysate
NBP2-07741
AP1M2 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol AP1M2