AP2 alpha Antibody (3B5)

Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Chicken.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.

Showing Publications 1 - 3 of 3.

Publications using NB100-74359	Applications	Species
Kim S, Morgunova E, Naqvi S et al. DNA-guided transcription factor cooperativity shapes face and limb mesenchyme bioRxiv 2023-06-02 [PMID: 37398193]
Wilson NR, Olm-Shipman AJ, Acevedo DS et al. SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination. Sci Rep. 2016-01-20 [PMID: 26787558] (ICC/IF, Mouse)	ICC/IF	Mouse
Milgrom-Hoffman M, Michailovici I, Ferrara N et al. Endothelial cells regulate neural crest and second heart field morphogenesis. Biol Open 2014-07-04 [PMID: 24996922] (IHC-Fr, Chicken) Details: Antibody used as cranial neural crest marker in IHC-Fr application on cryo-sections of controls and Flk1 cKO mutants chick embryos (Fig. 2M/R). Staining also performed on Mesp1Cre Flk1 control and mutant embryos/explants (Fig 3C/G).	IHC-Fr	Chicken

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-Fr	Mouse	06/23/2021	View Summary Application Immunohistochemistry-Frozen Sample Tested E9.5 mouse embryo fixed in 4% PFA Species Mouse Lot VG301904 Comments Comments Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 20ug/mL Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody dilution - 1:1000 Stained on a E9.5 mouse pharyngeal mesoderm region

Array NB100-74359

• AP2 alpha Antibodies
• AP2 alpha Lysate
• AP2 alpha Proteins