alpha Tubulin Antibody (YOL1/34) - BSA Free

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Drosophila, Fungi, Yeast.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 27. Show All 27 Publications. Collapse Publications.

Publications using NB100-1639	Applications	Species
Harry Warner, Giulia Franciosa, Guus van der Borg, Britt Coenen, Felix Faas, Claire Koenig, Rinse de Boer, René Classens, Sjors Maassen, Maksim V Baranov, Shweta Mahajan, Deepti Dabral, Frans Bianchi, Niek van Hilten, Herre Jelger Risselada, Wouter H Roos, Jesper Velgaard Olsen, Laia Querol Cano, Geert van den Bogaart Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation. Cell reports 2024-04-01 [PMID: 38416638]
Wang PH, Washburn RS, Mariuzza DL et al. Reciprocal transmission of activating and inhibitory signals and cell fate in regenerating T cells Cell reports 2023-09-26 [PMID: 37756164] (ICC/IF, Mouse)	ICC/IF	Mouse
Wang Y, Mao Y, Chen X et al. Homeostatic control of an iron repressor in a GI tract resident bioRxiv 2023-01-31 [PMID: 37227051] (WB)	WB
Stempels FC, Jiang M, Warner HM et al. Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters The Journal of cell biology 2023-07-03 [PMID: 37200023] (ICC/IF, Human)	ICC/IF	Human
Acharya BR, Fang JS, Jeffery ED et al. Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Life science alliance 2023-08-01 [PMID: 37197981]
Feng C, Kou L, Yin P, Jing Y Excessive activation of IL 33/ST2 in cancer associated fibroblasts promotes invasion and metastasis in ovarian cancer Oncology Letters 2022-03-17 [PMID: 35399326] (WB, Human)	WB	Human
Elfrink S, Ter Beest M, Janssen L et al. IRF8 is a transcriptional activator of CD37 expression in diffuse large B-cell lymphoma Blood advances 2022-01-27 [PMID: 35086136] (WB)	WB
Stempels FC, Janssens MH, Ter Beest M et al. Novel and conventional inhibitors of canonical autophagy differently affect LC3-associated phagocytosis FEBS letters 2022-01-10 [PMID: 35007347] (WB, Human)	WB	Human
Peter T. A. Linders, Eveline C. F. Gerretsen, Angel Ashikov, Mari-Anne Vals, Rinse de Boer, Natalia H. Revelo, Richard Arts, Melissa Baerenfaenger, Fokje Zijlstra, Karin Huijben, Kimiyo Raymond, Kai Muru, Olga Fjodorova, Sander Pajusalu, Katrin Õunap, Martin ter Beest, Dirk Lefeber, Geert van den Bogaart Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5 Nature Communications 2021-10-28 [PMID: 34711829]
Yuan T, Yihui Z, Zhujun C et al. A gain-of-function mutation in PDR1 of Candida glabrata decreases EPA1 expression and attenuates adherence to epithelial cells through enhancing recruitment of the Mediator subunit Gal11A Microbiological Research 2020-06-01 [PMID: 32563123] (WB)	WB
de Haas N, de Koning C et al. STAT Family Protein Expression and Phosphorylation State during moDC Development Is Altered by Platinum-Based Chemotherapeutics. J Immunol Res 2019-07-17 [PMID: 31309123] (WB, Human)	WB	Human
Liang C, Zhang B, Cui L et al. Mgm1 is required for maintenance of mitochondrial function and virulence in Candida albicans. Fungal Genet. Biol. 2018-09-18 [PMID: 30240789] (WB, Fungi)	WB	Fungi
Sondergaard Jonas Norskov, Van Heeringen Simon J, Looman Maaike W G et al. Dendritic Cells Actively Limit Interleukin-10 Production Under Inflammatory Conditions via DC-SCRIPT and Dual-Specificity Phosphatase 4. Frontiers in Immunology 2018-01-01 [PMID: 29988341] (WB, Human)	WB	Human
Tian Y, Gao N, Ni Q et al. Sequence modification of the master regulator Pdr1 interferes with its transcriptional autoregulation and confers altered azole resistance in Candida glabrata. FEMS Yeast Res. 2018-04-10 [PMID: 29648590] (WB)	WB
Agerholm M, Dall M, Jensen BAH et al. Perturbations of NAD salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle. Am. J. Physiol. Endocrinol. Metab. 2017-12-05 [PMID: 29208611] (Mouse)		Mouse
Huang X, Chen X, He Y et al. Mitochondrial complex I bridges a connection between regulation of carbon flexibility and gastrointestinal commensalism in the human fungal pathogen Candida albicans. PLoS Pathog. 2017-06-01 [PMID: 28570675] (WB, Human)	WB	Human
Newnham L, Jordan PW, Carballo JA et al. Ipl1/Aurora Kinase Suppresses S-CDK-Driven Spindle Formation during Prophase I to Ensure Chromosome Integrity during Meiosis. PLoS One 2013-12-27 [PMID: 24386320] (ICC/IF, Yeast)	ICC/IF	Yeast
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. A Meiosis-Specific Form of the APC/C Promotes the Oocyte-to-Embryo Transition by Decreasing Levels of the Polo Kinase Inhibitor Matrimony. PLoS Biol. 2013-09-01 [PMID: 24019759] (WB, Drosophila)	WB	Drosophila
Pande K, Chen C, Noble SM et al. Passage through the mammalian gut triggers a phenotypic switch that promotes Candida albicans commensalism. Nat Genet 2013-07-28 [PMID: 23892606] (WB, Fungi)	WB	Fungi
Ding X, Yu Q, Xu N et al. Ecm7, a regulator of HACS, functions in calcium homeostasis maintenance, oxidative stress response and hyphal development in Candida albicans. Fungal Genet Biol 2013-06-13 [PMID: 23769872] (WB, Yeast)	WB	Yeast
Chen C, Noble SM. Post-Transcriptional Regulation of the Sef1 Transcription Factor Controls the Virulence of Candida albicans in Its Mammalian Host PLoS Pathog 2012-11-01 [PMID: 23133381] (WB, Fungi)	WB	Fungi
Hwang WW, Madhani HD. Nonredundant requirement for multiple histone modifications for the early anaphase release of the mitotic exit regulator Cdc14 from nucleolar chromatin. PLoS Genet. 2009-08-01 [PMID: 19662160] (ICC/IF, Yeast)	ICC/IF	Yeast
Yu L, Lopez A, Anaflous A et al. Chemical-genetic profiling of imidazo[1,2-a]pyridines and -pyrimidines reveals target pathways conserved between yeast and human cells. PLoS Genet. 2008-11-01 [PMID: 19043571] (WB, Yeast)	WB	Yeast
Yamamoto A, Nunoshiba T, Umezu K et al. Phenyl hydroquinone, an Ames test-negative carcinogen, induces Hog1-dependent stress response signaling. FEBS J. 2008-11-01 [PMID: 18959758] (ICC/IF, Yeast)	ICC/IF	Yeast
Venkatesan, RN et al. Mutation at the Polymerase Active Site of Mouse DNA Polymerase delta Increases Genomic Instability Accelerates Tumorigenesis. Mol Cell Biol;27:7669-7682. [PMID: 17785453]
Byers HR, Dykstra SG, Boissel SJ. Requirement of dynactin p150 (Glued) subunit for the functional integrity of the keratinocyte microparasol. J Invest Dermatol. 2007-07-01 [PMID: 17344930] (ICC/IF, Human)	ICC/IF	Human
Nunoshiba T, Watanabe E, Takahashi T et al. Ames test-negative carcinogen, ortho-phenyl phenol, binds tubulin and causes aneuploidy in budding yeast Mutat Res. 2007-04-01 [PMID: 17289091] (ICC/IF, Yeast)	ICC/IF	Yeast
Show All 27 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	09/26/2014	View Summary Application Western Blot Sample Tested Jurkat and THP1 cells Species Human

Array NB100-1639

• alpha Tubulin Antibodies
• alpha Tubulin Lysate
• alpha Tubulin Proteins