![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (15 | 5 | 2 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0018.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (15 | 5 | 2 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Dr, Fi, Gt, Ze, Po, Bv, Pm, Rb, RM, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, Func |
| Clone | 114 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary. |
| Predicted Species | Porcine (100%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (95%), Primate (99%). Backed by our 100% Guarantee. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUBA1B |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol (pH7) |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Tubulin alpha-1B Antibody (NBP2-43837)Find related products by research area.
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| Gene Symbol | TUBA1B |
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0020.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0011.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0004.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0005.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0006.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0007.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted at a dilution of 1:10000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0013.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0015.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0001.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0008.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Green: alpha Tubulin antibody [114] diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0009.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Green: alpha Tubulin antibody [114] diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0017.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0002.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.")
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0003.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.")
![Tumorsphere: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Tumorsphere-NBP2-43837-img0019.jpg "Tumorsphere: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
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