![Western Blot: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin alpha-8 monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Western-Blot-NBP2-74709-img0002.jpg "Western Blot: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin alpha-8 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | OTI2G6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUBA8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 50.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Simple Western beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/16250.jpg)
![Immunocytochemistry beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/beta-III_Tubulin_MAB1195_Immunocytochemistry_6556.jpg)
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TUBA8 |
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-8.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74709-img0003.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-8.")
![Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded prostate tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74709-img0008.jpg "Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded prostate tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.")
![Western Blot: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubuli](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Western-Blot-NBP2-74709-img0001.jpg "Western Blot: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubuli")
![Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74709-img0004.jpg "Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.")
![Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74709-img0005.jpg "Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.")
![Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded Kidney tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74709-img0006.jpg "Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded Kidney tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.")
![Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded pancreas tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74709-img0007.jpg "Immunohistochemistry: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - Staining of paraffin-embedded pancreas tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.")
![Immunoprecipitation: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TUBA8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-8-Antibody-OTI2G6-Azide-and-BSA-Free-Immunoprecipitation-NBP2-74709-img0009.jpg "Immunoprecipitation: Tubulin alpha-8 Antibody (OTI2G6) - Azide and BSA Free [NBP2-74709] - (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TUBA8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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