![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249403851.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, Func |
| Clone | HL1478 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-term region of Human Dopamine D2R/DRD2. The exact sequence is proprietary. |
| Predicted Species | Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DRD2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Dopamine D2R/DRD2 Antibody (NBP3-25443)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DRD2 |
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled mouse tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084p) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-212024941490.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled mouse tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084p) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249415913.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249413358.jpg "Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Protein Array: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) specifically detects DRD2 protein on a VirD(TM)-GPCR array. Yellow: DRD2 White: DRD3 Blue: DRD5 Bottom left: Positive control](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249342745.jpg "Protein Array: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) specifically detects DRD2 protein on a VirD(TM)-GPCR array. Yellow: DRD2 White: DRD3 Blue: DRD5 Bottom left: Positive control")
![Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249435515.jpg "Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-212024941419.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249413362.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249383153.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249403855.jpg "Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249362783.jpg "Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249422687.jpg "Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-212024942266.jpg "Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")