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Musashi-1 Antibody - BSA Free

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Western Blot: Musashi-1 Antibody [NB100-1759] - Detection of Musashi-1 protein in 10 ug of rat neural stem cell lysate (lane 1) and normal human brain lysate (lane 2) with Musashi-1 antibody NB100-1759 at a dilution of ...read more
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NB100-1759] - Staining with Musashi polyclonal antibody (1:100 dilution) shows Musashi (green) staining on neural rosettes (human). Nuclei are counterstained ...read more
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NB100-1759] - Musashi-positive cells stained with Musashi antibody (1:150 dilution) in the ventricular zone of E14.5 mouse medulla. Image taken at 200x ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Musashi-1 Antibody - BSA Free Summary

Immunogen
Reacts with residues 5-21 [APQPGLASPDSPHDPCK] of the human, mouse and rat Musashi 1 protein.
Marker
Neuronal Stem Cell Marker
Specificity
Musashi 1 - Neuronal Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MSI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24006477)
Publications
Read Publications using
NB100-1759 in the following applications:

Reactivity Notes

Reacts with human, rat, and mouse Musashi 1 protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Musashi-1 Antibody - BSA Free

  • MSI1
  • Musashi (Drosophila) homolog 1
  • musashi homolog 1 (Drosophila)
  • Musashi1
  • Musashi-1
  • RNA-binding protein Musashi homolog 1

Background

Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Musashi-1 (Msi-1) has been shown to increase the accumulation of tau isoforms in intracellular inclusions in dementia. It may play a role in the pathogenesis of various neurodegenerative disorders. Musashi1 has also been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in cancer development. Msi-1 also appears to play be an vital role in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Musashi-1 Antibody (NB100-1759)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC.


Filter By Application
ICC/IF
(1)
IF/IHC
(3)
IHC
(1)
All Applications
Filter By Species
Human
(3)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-1759 Applications Species
El-Salhy M, Patcharatrakul T, Hatlebakk JG et al. Enteroendocrine, Musashi 1 and neurogenin 3 cells in the large intestine of Thai and Norwegian patients with irritable bowel syndrome Scand. J. Gastroenterol. 2017-08-30 [PMID: 28853300] (IF/IHC, Human) IF/IHC Human
El-Salhy M, Umezawa K, Hatlebakk JG, Gilja OH. Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced colitis Molecular Medicine Reports 2017-03-01 [PMID: 28259987] (IHC) IHC
El-Salhy M, Mazzawi T et al. Enteroendocrine cells, stem cells and differentiation progenitors in rats with TNBS-induced colitis. Int J Mol Med 2016-01-12 [PMID: 27779708] (IF/IHC, Rat) IF/IHC Rat
Nizzardo M, Simone C, Rizzo F et al. Minimally invasive transplantation of iPSC-derived ALDHhiSSCloVLA4+ neural stem cells effectively improves the phenotype of an amyotrophic lateral sclerosis model. Hum. Mol. Genet. 2014-01-15 [PMID: 24006477] (ICC/IF, Human) ICC/IF Human
Kanwar SS, Yu Y, Nautiyal J et al. The Wnt/beta-catenin pathway regulates growth and maintenance of colonospheres. Mol Cancer. 2010-08-06 [PMID: 20691072]

Details:
Musashi-1 (IMG-4982A). WB: HT-29, HCT-116, and HCT-116 p53 -/- cell lines, Fig 1B. Note: Musashi-1 was detected at 35 kDa. Regulation of autologous immunity to the mouse 5T4 oncofoetal antigen: implications for immunotherapy. 4APC (IMG-6217G).
Fishbein TM, Novitskiy G, Lough DM et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 2009-07-01 [PMID: 19519821] (IF/IHC, Human) IF/IHC Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Musashi-1 Antibody (NB100-1759) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSI1
Entrez