| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2029-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3797-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-56688SS | |||
| Azide Free | NB100-56688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8935-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2097-SP | |||
![TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2029_human-mouse-rat-smad7-mab-clone-293039-41202410484331.jpg)
![TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2029_human-mouse-rat-smad7-mab-clone-293039-4120241049448.jpg)
![Western Blot: ZEB2 Antibody [NBP1-82991] - Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-ZEB2 antibody. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-82991-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-82991] - Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-ZEB2 antibody. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-82991] - Depletion of TRIM14 increased ZEB2 polyubiquitination and proteasomal degradation. Western blot analysis of TRIM14, ZEB2 and beta-actin in LN229 and U251 cells transduced with the indicated shRNA in the absence or presence of 10uM MG132. Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-019-1070-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-82991-img0018.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0017.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0014.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human colon shows moderate nuclear positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0015.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human glioma shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0016.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Western-Blot-NBP1-77836-img0005.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - BH4 precursor, sepiapterin, normalizes NO level and ameliorates malignant phenotype of precancerous and cancerous cells. Representative images of the progression series cultivated in 3D ECM with or without sepiapterin (20 uM) and stained for phospho-SMAD3 or ERBB2. Nuclei were counterstained with DAPI (blue). Scale bars: 20 um. (Bottom) Quantification of phospho-SMAD3 (left) and ERBB2 levels (right) of the progression series. Error bars: mean +/- STDEV. *p < 0.05; **p < 0.01 and ***p < 0.001. Note that the effects of sepiapterin are only discernible in precancerous and cancerous cells, but not in non-malignant cells. (See the results of different concentrations of sepiapterin in Supplementary Fig. 8). Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-43239-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77836-img0007.jpg)
![Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Immunohistochemistry-NBP1-77836-img0006.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0005.jpg "Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP2-24710-img0004.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0001.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0003.jpg)
![Western Blot: SMAD6 Antibody [NB100-56440] - Analysis in mouse adult lung tissue. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SMAD6-Antibody-Western-Blot-NB100-56440-img0006.jpg "Western Blot: SMAD6 Antibody [NB100-56440] - Analysis in mouse adult lung tissue. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SMAD6 (NB100-56440) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SMAD6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56440-img0004.jpg)
![Immunohistochemistry-Paraffin: SMAD6 Antibody [NB100-56440] - SMAD6 was detected in immersion fixed paraffin-embedded sections of human lung using Rabbit Anti-Human SMAD6 polyclonal Antibody (Catalog # NB100-56440) at 5 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Nuclear staining was localized to the pneumocytes.](http://images.novusbio.com/fullsize/SMAD6-Antibody-Immunohistochemistry-Paraffin-NB100-56440-img0005.jpg)
![Western Blot: SMAD6 Antibody [NB100-56440] - Analysis using SMAD6 antibody. Lysate from Jurkat cells probed with SMAD6 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/SMAD6-Antibody-Western-Blot-NB100-56440-img0002.jpg)
![Simple Western: SMAD6 Antibody [NB100-56440] - Simple Western lane view shows a specific band for SMAD6 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SMAD6-Antibody-Simple-Western-NB100-56440-img0003.jpg)
![Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NB100-56479-img0008.jpg "Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56479-img0006.jpg)
![Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunohistochemistry-Paraffin-NB100-56479-img0004.jpg)
![Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at 0.5 ug/mL. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NB100-56479-img0005.jpg)
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HepG2.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0017.jpg "Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Immunocytochemistry-Immunofluorescence-H00057154-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Immunohistochemistry-Paraffin-H00057154-M01-img0013.jpg)
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in Raw 264.7 ( Cat # L024V1 ).](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0002.jpg)
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0003.jpg)
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HeLa.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0016.jpg)
![Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody (M01), clone 1D7.Lane 1: SMURF1 transfected lysate(83.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Western-Blot-H00057154-M01-img0018.jpg)
![Immunoprecipitation: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SMURF1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-Immunoprecipitation-H00057154-M01-img0014.jpg)
![ELISA: SMURF1 Antibody (1D7) [H00057154-M01] - Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMURF1-Antibody-1D7-ELISA-H00057154-M01-img0019.jpg)
![Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/SMAD4-Antibody-Western-Blot-NBP2-24951-img0001.jpg "Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24951-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunohistochemistry-Paraffin-NBP2-24951-img0002.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0024.jpg "Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Immunohistochemistry-Paraffin-H00004088-M02-img0017.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in HeLa.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0019.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in PC-12.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0020.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in 293.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0021.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in Jurkat.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0022.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in human colon.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0023.jpg)
![Proximity Ligation Assay: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Proximity-Ligation-Assay-H00004088-M02-img0015.jpg)
![ELISA: Smad3 Antibody (7F3) [H00004088-M02] - Detection limit for recombinant GST tagged SMAD3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-ELISA-H00004088-M02-img0025.jpg)