![Western Blot: Wnt-11 Antibody [NBP1-31406] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Western-Blot-NBP1-31406-img0018.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human WNT11. The exact sequence is proprietary. |
| Localization | Secreted, extracellular space, extracellular matrix |
| Predicted Species | Bovine (98%), Rabbit (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | WNT11 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Immunogen affinity purified |
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![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Wnt-11 Antibody (NBP1-31406)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31406-img0016.jpg "Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded mouse colon. WNT11 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunohistochemistry-Paraffin-NBP1-31406-img0012.jpg "Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded mouse colon. WNT11 antibody dilution: 1:500.")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - A. 50 ug mouse heart extract 10 % SDS-PAGE WNT11 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Western-Blot-NBP1-31406-img0013.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - A. 50 ug mouse heart extract 10 % SDS-PAGE WNT11 antibody dilution: 1:1000")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract12 % SDS-PAGE WNT11 antibody dilution: 1:100](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Western-Blot-NBP1-31406-img0014.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract12 % SDS-PAGE WNT11 antibody dilution: 1:100")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Western-Blot-NBP1-31406-img0017.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - Analysis of HeLa, using NBP1-31406 at 1:200 dilution.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31406-img0008.jpg "Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - Analysis of HeLa, using NBP1-31406 at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunohistochemistry-Paraffin-NBP1-31406-img0009.jpg "Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")