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Wnt-11 Antibodies

We offer Wnt-11 Antibodies for use in common research applications: Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Wnt-11 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Wnt-11 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Wnt-11 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Wnt-11 polyclonal antibodies and browse our Wnt-11 monoclonal antibody catalog.

Alternate Names for Wnt-11 Antibodies

anti-Wnt-11 antibody, anti-WNT11 antibody, anti-HWNT11 antibody, anti-MGC141946 antibody, anti-MGC141948 antibody, anti-protein Wnt-11 antibody, anti-wingless-type MMTV integration site family, member 11 antibody

6 Results for "wnt-11" in Primary Antibodies

AF2647

Wnt-11 Antibody [Unconjugated]

(4 Publications)

100 μg

AF2647

Wnt-11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Hypoxia induces expression of WNT11 through VHL.(A,B) Higher basal levels of WNT11 protein in Vhl-deleted cells (lenti-Cre infected Vhlf/f). EMSCs isolated from Vhlf/f mouse were infected with lentivirus carrying either GFP gene (for control) or Cre recombinase (for knockout). Non-infected cells were also used as a control. Immunoblot analysis of control or Vhl KO EMSCs treated with 0.1 mM DMOG (A), and EMSCs exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs (B). Laminin, alpha -tubulin, and lamin A/C were used as loading controls, WNT11 normalized to alpha -Tubulin was shown. (C,D) Inactivation of the Vhl gene results in increased Wnt11 mRNA. Wnt11 and Vegf mRNA levels in liver (C) or duodenum (D) were measured by qPCR in Liver-VhlcKO or duodenum-VhlcKO and control mice (n = 5 per group). Values normalized to Tbp mRNA are expressed relative to tissues from control mice. For panels (C,D), values are mean ± s.e.m. *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520), licensed under a CC-BY license. Not internally tested by R&D Systems.

WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO (0.1 mM) or DMOG (0.1 mM) for 24 hrs. Values were normalized to Tbp mRNA and are expressed relative to control (n = 3). (B,C) Increased Wnt11 mRNA by hypoxia in EMSC preadipocytes and adipocytes (Day 0–12 after differentiation) (B), and C2C12 myoblast and myocyte (Day 0 and 8 after differentiation) (C). Wnt11 mRNA was assessed by quantitative PCR in cells exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs. (n = 4). Values were normalized to Tbp mRNA and are expressed relative to 21% O2 samples (left panel). (D) Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. (E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) and 4T1 cells (F). (G) EMSCs treated with 0.1 mM DMOG for the indicated times. Wnt11 and Vegf mRNA expression was measured by qPCR and normalized to Tbp mRNA (n = 4). (H) WNT11 protein levels after DMOG treatment normalized to alpha -Tubulin (upper panel; n = 4). Representative immunoblots of EMSCs treated with 0.1 mM DMOG for the indicated times (Lower panel). (I) Protein expression in MDA-MB-231 cells treated with 0.1 mM DMOG. (J) Induction of Wnt11 promoter activity by hypoxia or hypoxia mimetics. pGL3-Wnt11 promoter plasmid was transfected into C2C12 cells. Cells were incubated with DMOG (left panel, n = 4) or under 21% O2 or 1% O2 (right panel, n = 8) for 24 hrs. For panels (A–C,G,H,J), values are mean ± s.e.m. *p < 0.05, **p < 0.01. For panels of immunoblotting, laminin, alpha -tubulin, and ERK were used as loading controls, WNT11 normalized to alpha -Tubulin was shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520), licensed under a CC-BY license. Not internally tested by R&D Systems.

WNT11 regulates MMPs activities.(A–C) (Top panels): Serum-free medium was conditioned for 24 hrs by the indicated cells, concentrated 20-fold and assayed by gelatin zymography. Gelatinolytic activity is indicated by clear zones against a dark background of stained substrate. (Bottom): Whole cell extracts were immunoblotted with indicated antibodies. (A) Overexpression of Wnt11 in EMSC or BT473 cells enhances activity of MMP-9 and MMP-2. (B) Impaired activity of MMP-9 and MMP-2 in MDA-MB-231 cells (left) or EMSCs (right) stably expressing Wnt11 shRNAs and treated with DMOG. (C) WNT11 is required for MMP-9 and MMP-2 activity in MDA-MB-231 cells (left) or EMSCs (right) under normoxic and hypoxic culture conditions. (D) WNT11 regulates MMP2 protein in media. (Top): conditioned media from indicated cells and treatments. (Bottom): whole cell lysates were immunoblotted with indicated antibodies. (E) Recombinant WNT11 induces both MMP-2 protein and MMP-2 activity in media. (Top panels): Gelatin zymography and immunoblot of serum-free medium conditioned for the indicated times after recombinant WNT11 (r-WNT11) treatment. (Bottom): Whole cell lysates were immunoblotted with indicated antibodies. (F) MMP-2 inhibitor attenuated induced migration by WNT11. MDA-MB-231 cells infected with lentiviruses for stable expression of Wnt11 or GFP (n = 4) were incubated with either vehicle or 1 μM of ARP100. Media in the lower compartment had same concentration of DMSO or inhibitor. Values are mean ± s.e.m. *p < 0.05, **p < 0.01. For panels (A–D), HIF-1 alpha and HIF-2 alpha were shown as a marker of hypoxia, WNT11 normalized to alpha -Tubulin was shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520), licensed under a CC-BY license. Not internally tested by R&D Systems.

Induced WNT11 expression with tumor hypoxia and WNT11 regulates tumor growth.Antiangiogenic therapy induces WNT11 expression in the orthotopic malignant glioma model. Athymic mice implanted with U87 delta EGFR cells were administered either bevacizumab (6 mg/kg) or vehicle three times per week for 4 weeks. (A) Increased Wnt11 mRNA in xenografts from mice treated with bevacizumab. Values were normalized to HPRT mRNA and are expressed relative to control (n = 10 per group). Values are mean ± s.e.m. *p < 0.05, **p < 0.01. (B) Bevacizumab increased expression of HIF-1 alpha and HIF-2 alpha and WNT11. First 10 lanes are control tumors, and the last 10 lanes are tumors from bevacizumab-treated animals. Lysates from whole tissue and nuclei are indicated. alpha -Tubulin, actin and lamin A/C are loading controls. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520), licensed under a CC-BY license. Not internally tested by R&D Systems.

HIF-1 alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia.(A,C–E) EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. (A) Attenuated WNT11 expression in Hif-1 alpha KO EMSCs (lenti-Cre infected Hif-1af/f). (B) HIF-1 alpha regulates WNT11 expression during hypoxia. Impaired WNT11 expression in MDA-MB-231 cells stably expressing HIF-1 alpha shRNAs with hypoxia. Cells were exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs (left panel). Overexpression of HIF-1 alpha in MDA-MB-231 cells enhances WNT11 expression (right panel). (C) Markedly increased WNT11 levels in Hif-1 alpha overexpressing EMSCs (lenti-Cre infected Hif-1 alpha -Tgfl-Stop). (D) Elevated HIF-1 alpha and WNT11 protein after DMOG treatment in HIF-2 alpha KO cells (lenti-Cre infectied Hif-2 alpha fl/fl). (E) Little effect of HIF-2 alpha overexpression on WNT11 protein expression in EMSC (lenti-Cre infectied Hif-2 alpha -Tgfl-Stop). (F) HIF-1 alpha binds to WNT11 and VEGF promoters as assessed by ChIP analyses (n = 3 each condition). (G) WNT11 expression was suppressed in cells with a beta -catenin deficiency. EMSCs stably expressing shRNAs against beta -catenin or scrambled control were treated with 0.1 mM DMOG for 24 hrs and analyzed by immunoblotting. (H) Co-transfection with expression vectors for beta -catenin, Hif-1 alpha and ARNT stimulates further induction of Wnt11 promoter activity. Constructs encoding Hif-1 alpha , Hif-2 alpha , ARNT, beta -catenin and Wnt11 promoter-luciferase reporter, were transiently transfected into HEK293T cells. Cells were harvested and luciferase activities were measured 48 hrs after transfection (n = 3). For panels (A–E,G), laminin, alpha -tubulin, ERK1/2 and lamin A/C were used as loading controls, WNT11 normalized to alpha -Tubulin was shown under the bots. For panels (F,H), values are mean ± s.e.m. *p < 0.05, **p < 0.01 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2647-SP

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NBP1-31406

Wnt-11 Antibody

(1 Publication)

100 ul

NBP1-31406

Western Blot: Wnt-11 Antibody [NBP1-31406] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded mouse colon. WNT11 antibody dilution: 1:500.

Western Blot: Wnt-11 Antibody [NBP1-31406] - A. 50 ug mouse heart extract 10 % SDS-PAGE WNT11 antibody dilution: 1:1000

Western Blot: Wnt-11 Antibody [NBP1-31406] - A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract12 % SDS-PAGE WNT11 antibody dilution: 1:100

Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - Analysis of HeLa, using NBP1-31406 at 1:200 dilution.

Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.

Reactivity:

Hu, Mu, Rt, Bv, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Rb

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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MAB3746

Wnt-11 Antibody (310622)

100 μg

MAB3746

Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3746-SP

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NBP3-25750

Wnt-11 Antibody (HL1630) - Azide and BSA Free

100 ul

NBP3-25750

Western Blot: Wnt-11 Antibody (HL1630) - Azide and BSA Free [NBP3-25750] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody [HL1630] (NBP3-25750) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-11 Antibody (HL1630) - Azide and BSA Free [NBP3-25750] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody [HL1630] (NBP3-25750) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-11 Antibody (HL1630) - Azide and BSA Free [NBP3-25750] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody [HL1630] (NBP3-25750) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Rb

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-57600

Wnt-11 Antibody

(1 Publication)

100 ul

NBP2-57600

Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP2-57600] - Staining of human cell line HaCaT shows localization to cytosol & centrosome. Antibody staining is shown in green.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-13523

Wnt-11 Antibody

0.1 ml

NBP2-13523

Immunohistochemistry-Paraffin: Wnt11 Antibody [NBP2-13523] Staining of human stomach, upper shows strong cytoplasmic positivity in a subset of glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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