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WASF1/WAVE1 Antibody

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A172 human glioblastoma cell line, and 293T human embryonic kidney cell line, and mouse brain tissue. PVDF membrane was probed with 1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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WASF1/WAVE1 Antibody Summary

Immunogen
E. coli-derived recombinant human WASF1/WAVE1
Met1-Tyr250
Accession # Q92558
Specificity
Detects endogenous human and mouse WASF1/WAVE1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WASF1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5514 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WASF1/WAVE1 Antibody

  • KIAA0269WAVEhomology of dictyostelium scar 1
  • Protein WAVE-1
  • SCAR1
  • SCAR1WASP family protein member 1
  • Verprolin homology domain-containing protein 1
  • WAS protein family, member 1
  • WASF1
  • WASP1
  • WAVE1
  • WAVE1FLJ31482
  • wisk

Background

WASF1/WAVE1 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 1) is an 80‑85 kDa member of the SCAR/WAVE family of proteins. It is expressed in diverse cell types such as neurons, fibroblasts, macrophages, platelets, oligodendroglia and breast epithelium. WASF1/WAVE1 is normally inactive, being phosphorylated at Ser310 and complexed with HSPC300, PIR121 and Nap125. Upon activation, it is dephosphorylated and decoupled, heterodimerizes with WASP, and initiates actin polymerization. Human WASF1/WAVE1 is 559 amino acids (aa) in length. It contains five sequential poly-Pro regions (aa 278‑435) and one VPH domain (aa 497‑514) that binds to actin and Arp2/3. There is one isoform that shows a 23 aa N-terminal extension. In mouse, a 60 kDa proteolytic cleavage form is reported. Over aa 1‑250, human WASF1/WAVE1 shares 99% aa identity with mouse WASF1/WAVE1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WASF1/WAVE1 Antibody (AF5514)(2)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol WASF1
Uniprot