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VEGF Antibody [Unconjugated] - 164

 (128 Publications)
 (2 Reviews)
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Western blot shows 25 ng of Recombinant Human VEGF165(Catalog # 293-VE), Recombinant Mouse VEGF164(Catalog # 493-MV), Recombinant Mouse VEGF120(Catalog # 494-VE), Recombinant Mouse VEGF-B167(Catalog # 2595-VE), Recombinant Mouse VEGF-B 186 (Catalog # 767-VE), Recombinant Mouse VEGF188(Catalog # 7916-MV), and Recombinant Rat VEGF164(Catalog # 564-RV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 18-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 25 ng of Recombinant Human VEGF165(Catalog # 293-VE), Recombinant Mouse VEGF164(Catalog # 493-MV), Recombinant Mouse VEGF120(Catalog # 494-VE), Recombinant Mouse VEGF-B167(Catalog # 2595-VE), ...read more
Vascular Endothelial Growth Factor 164 (VEGF164) was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) at 15 ...read more
Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by ...read more
Altered expression of VEGF-A following myeloid-specific ablation of NG2. Double immunostaining for VEGF-A (red) and CD31 (green) was used to quantify and localize VEGF-A expression in tumors from control (A–C) and ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF493
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-493-SP
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VEGF Antibody [Unconjugated] - 164 Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164
Ala27-Arg190
Accession # AAA40547
Specificity
Detects mouse VEGF in ELISAs and Western blots. In Western blots, detection of recombinant mouse (rm) VEGF164, rmVEGF120, rmVEGF188, and recombinant rat VEGF164 is observered but no cross-reactivity with rmVEGF-B167,rmVEGF-B186 , or recombinant human (rh) VEGF165 is observed. In sandwich immunoassays, less than 0.04% cross-reactivity with rhVEGF121, rhVEGF165, and rhVEGF/PlGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VEGFA
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization <0.15 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse VEGF164 Antibody (Catalog # AF-493-NA)
ELISA Detection: Mouse VEGF Biotinylated Antibody (Catalog # BAF493)
Standard:Recombinant Mouse VEGF164 (Catalog # 493-MV)
Reviewed Applications
Read 2 Reviews rated 3.5
using
AF-493-NA in the following applications:
Publications
Read Publications using
AF-493-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody [Unconjugated] - 164

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGF Antibody
[Unconjugated] - 164
AF-493-NA
VEGF Antibody [Unconjugated] - 164
Species: Mu
Applications: WB, IHC, B/N, ELISA(Cap)

Publications for VEGF Antibody (AF-493-NA)(128)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 14 applications: Blocking, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Functional Assay, ICC, IHC, IHC-Fr, IHC-P, In Vivo, Luminex Development, Neutralization, Western Blot.


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Blocking
(1)
ELISA Capture
(1)
ELISA Development
(3)
ELISA Development (Capture)
(1)
ELISA Development (Detection)
(1)
Functional Assay
(2)
ICC
(2)
IHC
(21)
IHC-Fr
(5)
IHC-P
(7)
In Vivo
(2)
Luminex Development
(1)
Neutralization
(45)
Western Blot
(10)
All Applications
Filter By Species
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(5)
Mouse
(86)
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(1)
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Showing Publications 1 - 10 of 128. Show All 128 Publications.
Publications using AF-493-NA Applications Species
Furue, A;Hattori, K;Hosono, K;Tanabe, M;Sato, E;Honda, M;Sekiguchi, K;Ito, Y;Majima, M;Narumiya, S;Kato, K;Amano, H; Inhibition of TP signaling promotes endometriosis growth and neovascularization Molecular medicine reports 2023-10-01 [PMID: 37654213] (IHC, Mouse) IHC Mouse
Michail, A;Gkikas, D;Stellas, D;Kaltezioti, V;Politis, PK; Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression Cells 2023-07-17 [PMID: 37508533] (ICC, Mouse) ICC Mouse
Damon-Soubeyrand C, Bongiovanni A, Chorfa A et al. Three-dimensional imaging of vascular development in the mouse epididymis eLife 6/13/2023 [PMID: 37310207]
Denninger, JK;Miller, LN;Walters, AE;Hosawi, M;Sebring, G;Rieskamp, JD;Ding, T;Rindani, R;Chen, KS;Senthilvelan, S;Volk, A;Zhao, F;Askwith, C;Kirby, ED; Neural stem and progenitor cells support and protect adult hippocampal function via vascular endothelial growth factor secretion bioRxiv : the preprint server for biology 2023-04-24 [PMID: 37163097] (IHC, Mouse) IHC Mouse
Liu S, Hur YH, Cai X et al. A tissue injury sensing and repair pathway distinct from host pathogen defense Cell 4/24/2023 [PMID: 37098344]
JH Wei, WJ Lee, JL Luo, HL Huang, SC Wang, RH Chou, PH Huang, SJ Lin Vertical Sleeve Gastrectomy Offers Protection against Disturbed Flow-Induced Atherosclerosis in High-Fat Diet-Fed Mice International Journal of Molecular Sciences, 2023-03-16;24(6):. 2023-03-16 [PMID: 36982743] (Western Blot, Human) Western Blot Human
E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone Experimental & Molecular Medicine, 2023-02-24;55(2):470-484. 2023-02-24 [PMID: 36828931] (IHC, Mouse) IHC Mouse
J Huang, Y Mao, Q Li, H Hong, N Tang, X Kang, Y Huang, J Liu, Q Gong, Y Yao, L Li Kallistatin prevents ovarian hyperstimulation syndrome by regulating vascular leakage Journal of Cellular and Molecular Medicine, 2022-07-21;26(16):4613-4623. 2022-07-21 [PMID: 35866203] (Neutralization, Mouse) Neutralization Mouse
Shogo Tamura, Masato Mukaide, Yumi Katsuragi, Wataru Fujii, Koya Odaira, Nobuaki Suzuki et al. Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development Journal of Biological Chemistry 5/1/2022 [PMID: 35304101]
J Fu, T Shinjo, Q Li, R St-Louis, K Park, MG Yu, H Yokomizo, F Simao, Q Huang, IH Wu, GL King Regenerating glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy JCI Insight, 2022-03-08;0(0):. 2022-03-08 [PMID: 35133981] (Neutralization, Mouse) Neutralization Mouse
Show All 128 Publications.

Reviews for VEGF Antibody (AF-493-NA) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF-493-NA:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23670941
SpeciesMouse
  3
reviewed by:
Verified Customer
 IF Mouse 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedPrimary mouse cortical astrocytes
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VEGF Antibody (AF-493-NA). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Secondary Antibodies

 

Isotype Controls

Additional VEGF Products

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Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.
Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.
Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.
Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.
mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.
Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.
Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.
The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.
The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.
The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.
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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0
 
 Verified Customer
01/08/2015
Application: WB
Species: Mouse
read this review
 
 Verified Customer
12/19/2014
Application: IF
Species: Mouse
read this review

Bioinformatics

Gene Symbol VEGFA
Uniprot