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VEGF Antibody [Unconjugated] - 164

Images

 
Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF 165Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room ...read more
VEGF was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 3 µg/mL for 1 hour ...read more
Recombinant Human VEGF165 (293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is ...read more
VEGF expression determined by Western blot and immunoprecipitation.A. Western blot using LiCor Odyssey to simultaneously image pan-VEGF and VEGF-A165b probed western blot. Two different podocyte samples, and a primary ...read more
Inhibitor of differentiation 4 (ID4) expression in breast cancer cells leads to the activation of an angiogenic programme in macrophages. a Expression matrix representing a panel of angiogenic factors evaluated using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, Dual ISH-IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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VEGF Antibody [Unconjugated] - 164 Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
Ala27-Arg191
Accession # NP_001165097.1
Specificity
Detects human VEGF in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VEGFA
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 0.02-0.12 ug/mL
  • Western Blot 5-10 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF-293-NA in the following applications:

Publications
Read Publications using
AF-293-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody [Unconjugated] - 164

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF Antibody (AF-293-NA)(85)

We have publications tested in 10 confirmed species: Human, Mouse, Avian - Quail, Bovine, Chicken, Guinea Pig, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Rabbit.

We have publications tested in 16 applications: Affinity Assay, Bioassay, Column, ELISA, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), ICC, IHC, IHC-Fr, IHC-P, Immunoassay Development, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Affinity Assay
(1)
Bioassay
(1)
Column
(1)
ELISA
(1)
ELISA Capture
(1)
ELISA Development
(8)
ELISA Development (Capture)
(1)
ELISA Development (Detection)
(1)
ICC
(4)
IHC
(3)
IHC-Fr
(1)
IHC-P
(4)
Immunoassay Development
(1)
Immunoprecipitation
(1)
Neutralization
(16)
Western Blot
(14)
All Applications
Filter By Species
Human
(41)
Mouse
(5)
Avian - Quail
(1)
Bovine
(1)
Chicken
(1)
Guinea Pig
(1)
Porcine
(3)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rabbit
(1)
Transgenic Rabbit
(1)
All Species
Showing Publications 1 - 10 of 85. Show All 85 Publications.
Publications using AF-293-NA Applications Species
Li, R;Weng, X;Hu, X;Wang, J;Zheng, L; Pigment epithelium?derived factor inhibits proliferation, invasion and angiogenesis, and induces ferroptosis of extravillous trophoblasts by targeting Wnt???catenin/VEGF signaling in placenta accreta spectrum Molecular medicine reports 2024-05-01 [PMID: 38488028] (Western Blot, Human) Western Blot Human
Quan, B;Li, Z;Yang, H;Li, S;Yan, X;Wang, Y; The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF165 and downregulating VEGF165b Heliyon 2023-08-01 [PMID: 37554848] (Western Blot, Human) Western Blot Human
Pedersen, T;Fojan, P;Pedersen, AKN;Magnusson, NE;Gurevich, L; Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays Biosensors 2023-05-05 [PMID: 37232880] (ELISA Capture, Human) ELISA Capture Human
O Benchama, MS Malamas, K Praveen, EC Ethier, MK Williams, A Makriyanni, HK Avraham Inhibition of triple negative breast cancer-associated inflammation and progression by N- acylethanolamine acid amide hydrolase (NAAA) Scientific Reports, 2022-12-23;12(1):22255. 2022-12-23 [PMID: 36564457] (IHC, Human) IHC Human
WJ Brewer, AM Xet-Mull, A Yu, MI Sweeney, EM Walton, DM Tobin Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection Cell Reports, 2022-12-13;41(11):111817. 2022-12-13 [PMID: 36516756] (ICC, Human) ICC Human
GL Lehmann, M Ginsberg, DJ Nolan, C Rodríguez, J Martínez-G, S Zeng, AP Voigt, RF Mullins, S Rafii, E Rodriguez-, I Benedicto Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells Cells, 2022-09-24;11(19):. 2022-09-24 [PMID: 36230937] (Neutralization, Human) Neutralization Human
H Han, Y Kim, H Mo, SH Choi, K Lee, YA Rim, JH Ju Preferential stimulation of melanocytes by M2 macrophages to produce melanin through vascular endothelial growth factor Scientific Reports, 2022-04-19;12(1):6416. 2022-04-19 [PMID: 35440608] (Bioassay, Human) Bioassay Human
P Li, H Xin, L Lu Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB Journal of Translational Medicine, 2021-01-06;19(1):4. 2021-01-06 [PMID: 33407591] (Western Blot, Human) Western Blot Human
JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells International Journal of Molecular Sciences, 2020-12-30;22(1):. 2020-12-30 [PMID: 33396613] (Western Blot, Human) Western Blot Human
D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&amp;alpha and VEGF expression Mol Med Rep, 2020-11-20;23(1):. 2020-11-20 [PMID: 33215223] (Neutralization, Western Blot, Human) Neutralization, Western Blot Human
Show All 85 Publications.

Reviews for VEGF Antibody (AF-293-NA) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF-293-NA:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot VEGF AF-293-NA
Enlarge
4
reviewed by:
Verified Customer
WB Human 02/25/2020
View

Summary

ApplicationWestern Blot
Sample TestedSkeletal muscle
SpeciesHuman
LotNA
  5
reviewed by:
Verified Customer
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22897854
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VEGF Antibody (AF-293-NA). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Secondary Antibodies

 

Isotype Controls

Additional VEGF Products

Blogs on VEGF. Showing 1-10 of 23 blog posts - Show all blog posts.

Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Showing 1-10 of 23 blog posts - Show all blog posts.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Verified Customer
02/25/2020
Application: WB
Species: Human

 
Verified Customer
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol VEGFA
Uniprot