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UBE2J1 Antibody (OTI3D1) - Azide and BSA Free

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Western Blot: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2J1.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human prostate tissue.
Flow Cytometry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Jurkat cells, using UBE2J1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Adenocarcinoma of Human breast tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Carcinoma of Human lung tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human breast tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human Kidney tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human liver tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human lung tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human lymphoma tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human pancreas tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Human tonsil tissue.
Flow Cytometry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Hela cells, using UBE2J1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI3D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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UBE2J1 Antibody (OTI3D1) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human UBE2J1(NP_057105) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
UBE2J1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500-2000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for UBE2J1 Antibody (OTI3D1) - Azide and BSA Free

  • CGI-76
  • EC 6.3.2.19
  • HSPC153
  • HSPC205
  • HSU93243
  • HsUBC6e
  • MGC12555
  • NCUBE1
  • NCUBE-1
  • NCUBE1HSUBC6e
  • Non-canonical ubiquitin-conjugating enzyme 1
  • non-canonical ubquitin conjugating enzyme 1
  • UBC6e
  • Ubc6p
  • UBE2J1
  • ubiquitin-conjugating enzyme E2 J1
  • ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)
  • Yeast ubiquitin-conjugating enzyme UBC6 homolog E

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBE2J1