![Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Western-Blot-NBP1-04351-img0006.jpg "Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | k1H3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant human UbcH8/Ube2L6 (1-152aa) purified from E. coli |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UBE2L6 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for UbcH8/Ube2L6 Antibody (NBP1-04351)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Immunocytochemistry-Immunofluorescence-NBP1-04351-img0008.jpg "Immunocytochemistry/Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Immunohistochemistry-Paraffin-NBP1-04351-img0001.jpg "Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)")
![Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Western-Blot-NBP1-04351-img0002.jpg "Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2L6 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Western-Blot-NBP1-04351-img0004.jpg "Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2L6 Transfected 293T cell lysate")
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