Tyrosine Hydroxylase Antibody (779427)

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

TH (Tyrosine 3-hydroxylase), also known as Tyrosine 3-monooxygenase, is a 60-62 kDa member of the biopterin-dependent aromatic amino acid hydroxylase family of molecules. It is expressed by neurons of the dopamine and autonomic nervous system, plus the neuroendocrine cells of the adrenal medulla. TH is considered the rate limiting enzyme for catecholamine synthesis, and serves to catalyze the hydroxylation of L-tyrosine. It maintains stores of catecholamines following secretion, and its activity is regulated by targeted site phosphorylation. Human TH is 528 amino acids (aa) in length. It contains an N-terminal ACT domain (aa 69-190) that binds small molecules and regulates enzyme activity, and a C-terminal enzymatic region (aa 196-493). There are three significant utilized phosphorylation sites. Two at Ser31 and Ser40 increase enzyme activity, while phosphorylation at Ser19 promotes subsequent Ser40 phosphorylation. TH functions as a 240 kDa noncovalent homotetramer. There are four potential splice variants. One shows a deletion of aa 31-34, a second shows a deletion of aa 31-61, while a third contains a Met substitution for aa 1-34. A fourth isoform variant shows a deletion of aa 35-61. Over aa 278-401, human TH shares 94% aa sequence identity with mouse TH, a molecule that most closely resembles the fourth human isoform variant described above.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IF, IHC, Western Blot.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using MAB7566	Applications	Species
He S, Liu J, Xue Y et al. Sympathetic Nerves Coordinate Corneal Epithelial Wound Healing by Controlling the Mobilization of Ly6Chi Monocytes From the Spleen to the Injured Cornea Investigative ophthalmology & visual science 9/1/2023 [PMID: 37682569]
W Dai, M Yang, P Xia, C Xiao, S Huang, Z Zhang, X Cheng, W Li, J Jin, J Zhang, B Wu, Y Zhang, PH Wu, Y Lin, W Wu, H Zhao, Y Zhang, WJ Lin, X Ye A functional role of meningeal lymphatics in sex difference of stress susceptibility in mice Nature Communications, 2022-08-16;13(1):4825. 2022-08-16 [PMID: 35974004] (IF, Mouse)	IF	Mouse
Panicker N, Kam TI, Wang H et al. Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease Neuron 5/25/2022 [PMID: 35654037]
Christopher R. Apostol, Kelsey Bernard, Parthasaradhireddy Tanguturi, Gabriella Molnar, Mitchell J. Bartlett, Lajos Szabò et al. Design and Synthesis of Brain Penetrant Glycopeptide Analogues of PACAP With Neuroprotective Potential for Traumatic Brain Injury and Parkinsonism Front Drug Discov (Lausanne) 3/3/2022 [PMID: 35237767]
MA Fernandez-, D Reyes-Coro, YM Flores-Mar, R Nadella, MJ Bannon, L Escobedo, M Maldonado-, J Santoyo-Sa, LO Soto-Rojas, C Luna-Herre, J Ayala-Davi, JA Gonzalez-B, G Flores, ME Gutierrez-, AJ Espadas-Al, IA Martínez-D, P Nava, D Martinez-F Cerebral dopamine neurotrophic factor transfection in dopamine neurons using neurotensin-polyplex nanoparticles reverses 6-hydroxydopamine-induced nigrostriatal neurodegeneration Neural regeneration research, 2022-04-01;17(4):854-866. 2022-04-01 [PMID: 34472486] (IHC, Rat)	IHC	Rat
Jasso KR, Kamba TK, Zimmerman AD et al. An N-terminal fusion allele to study melanin concentrating hormone receptor 1 genesis 8/1/2021 [PMID: 34124835]
S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization Cell Stem Cell, 2021-06-07;0(0):. 2021-06-07 [PMID: 34102139] (IHC, Mouse)	IHC	Mouse
S Chen, Y Han, L Yang, T Kim, M Nair, O Harschnitz, P Wang, J Zhu, SY Koo, X Tang, L Lacko, V Chandar, Y Bram, T Zhang, W Zhang, F He, J Caicedo, Y Huang, T Evans, P van der Va, MJ Titulaer, JKH Spoor, RL Furler, P Canoll, J Goldman, S Przedborsk, R Schwartz, D Ho, L Studer SARS-CoV-2 Infection Causes Dopaminergic Neuron Senescence Research square, 2021-05-21;0(0):. 2021-05-21 [PMID: 34031650] (IHC, Human)	IHC	Human
Daiqiang Liu, Jiayan Li, Jiayi Wu, Jiaqi Dai, Xinfeng Chen, Yujie Huang et al. Monochromatic Blue Light Activates Suprachiasmatic Nucleus Neuronal Activity and Promotes Arousal in Mice Under Sevoflurane Anesthesia Frontiers in Neural Circuits 8/18/2020 [PMID: 32973462]
Yan Fan, Fei Zeng, Russell W. Brown, Jennifer B. Price, Thomas C. Jones, Meng-Yang Zhu Transcription factors Phox2a/2b upregulate expression of noradrenergic and dopaminergic phenotypes in aged rat brains. Neurotoxicity Research 10/1/2020 [PMID: 32617854]
Lin M W, Lin C C et al. Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy. Antioxidants (Basel) 12/31/2019 [PMID: 31906147]
BE Kim, SW Choi, JH Shin, JJ Kim, I Kang, BC Lee, JY Lee, MG Kook, KS Kang Single-Factor SOX2 Mediates Direct Neural Reprogramming of Human Mesenchymal Stem Cells via Transfection of In Vitro Transcribed mRNA Cell Transplant, 2018-06-18;0(0):9636897187718. 2018-06-18 [PMID: 29909688] (ICC, Human)	ICC	Human
D Little, C Luft, O Mosaku, M Lorvellec, Z Yao, S Paillusson, J Kriston-Vi, S Gandhi, AY Abramov, R Ketteler, MJ Devine, P Gissen A single cell high content assay detects mitochondrial dysfunction in iPSC-derived neurons with mutations in SNCA Sci Rep, 2018-06-13;8(1):9033. 2018-06-13 [PMID: 29899557] (ICC, Human)	ICC	Human
E Leem, HJ Kim, M Choi, S Kim, YS Oh, KJ Lee, YS Choe, JY Um, WH Shin, JY Jeong, BK Jin, DW Kim, C McLean, PB Fisher, N Kholodilov, KS Ahn, JM Lee, UJ Jung, SG Lee, SR Kim Upregulation of neuronal astrocyte elevated gene-1 protects nigral dopaminergic neurons in vivo Cell Death Dis, 2018-05-01;9(5):449. 2018-05-01 [PMID: 29670079] (Western Blot, Human)	Western Blot	Human
J Schommer, G Marwarha, T Schommer, T Flick, J Lund, O Ghribi 27-Hydroxycholesterol increases ?-synuclein protein levels through proteasomal inhibition in human dopaminergic neurons BMC Neurosci, 2018-04-03;19(1):17. 2018-04-03 [PMID: 29614969] (ICC, Human)	ICC	Human
Lin JG, Chen CJ, Yang HB et al. Electroacupuncture Promotes Recovery of Motor Function and Reduces Dopaminergic Neuron Degeneration in Rodent Models of Parkinson's Disease Int J Mol Sci 8/24/2017 [PMID: 28837077]
Ahmed M. Abu El-Asrar, Mohammad Mairaj Mairaj Siddiquei, Mohd Imtiaz Nawaz, Karel Geboes, Ghulam Mohammad The Proinflammatory Cytokine High-Mobility Group Box-1 Mediates Retinal Neuropathy Induced by Diabetes Mediators of Inflammation 3/10/2014 [PMID: 24733965]
Show All 17 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Rat	09/02/2015	View Summary Application Immunohistochemistry-Frozen Sample Tested Frozen tissue sections or rat cerebrum Species Rat Lot 2458991

Array MAB7566

• Tyrosine Hydroxylase Antibodies
• Tyrosine Hydroxylase ELISA Kits
• Tyrosine Hydroxylase Lysate
• Tyrosine Hydroxylase Proteins