![Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Western-Blot-NBP2-37364-img0004.jpg "Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, KD |
| Clone | 10E4E6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Twist-1 (AA: 9-74) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TWIST1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody is CyTOF ready. Use in Knockdown Validated reported in scientific literature (PMID: 31113881). |
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| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Human | 09/27/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Twist-1 Antibody (NBP2-37364)Find related products by research area.
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Autophagy and Metastasis By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes... Read full blog post. |
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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| Gene Symbol | TWIST1 |
![Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of HeLa cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunocytochemistry-NBP2-37364-img0011.jpg "Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of HeLa cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - IHC staining of paraffin-embedded squamous cell carcinoma sample using Twist-1 antibody at 1:100 dilution. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunohistochemistry-Paraffin-NBP2-37364-img0018.jpg "Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - IHC staining of paraffin-embedded squamous cell carcinoma sample using Twist-1 antibody at 1:100 dilution. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0014.jpg "Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.")
![Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. Expected MW is 31.9 kDa.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Western-Blot-NBP2-37364-img0002.jpg "Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. Expected MW is 31.9 kDa.")
![Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Western-Blot-NBP2-37364-img0003.jpg "Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunohistochemistry-NBP2-37364-img0007.jpg "Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.")
![Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunohistochemistry-NBP2-37364-img0009.jpg "Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.")
![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0006.jpg "Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).")
![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0012.jpg "Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.")
![Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Intracellular-NBP2-37364-img0013.jpg "Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.")
![ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-ELISA-NBP2-37364-img0001.jpg "ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images2/RUNX2_MAB2006_Immunocytochemistry_13309.jpg)
![Western Blot Snail Antibody [Unconjugated]](https://images.novusbio.com/images2/Snail_AF3639_Western_Blot_5320.jpg)
![Intracellular Staining by Flow Cytometry Snail Antibody [Unconjugated]](https://images.novusbio.com/images2/Snail_AF3639_Flow_Cytometry_20834.jpg)
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![Flow Cytometry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)](https://images.novusbio.com/images2/mab7662_human-fgf-r3-iiic-mab-clone-136312-flow-cytometry-2112202112199.jpg)
![Immunocytochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)](https://images.novusbio.com/images2/mab7662_human-fgf-r3-iiic-mab-clone-136312-immunocytochemistry-186202172236.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
