![Western Blot: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. Endogenous VIM pulls down endogenous GIG. Indicated lysates were subjected to immunoprecipitation with either VIM antibody or control IgG (used as the mock condition throughout), and the membrane was probed sequentially for endogenous GIG and then VIM (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Western-Blot-NBP1-49924-img0002.jpg "Western Blot: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. Endogenous VIM pulls down endogenous GIG. Indicated lysates were subjected to immunoprecipitation with either VIM antibody or control IgG (used as the mock condition throughout), and the membrane was probed sequentially for endogenous GIG and then VIM (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 150 and 200 of human Gigaxonin using the numbering given in entry NP_071324.1 (GeneID 8139). |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GAN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot MAP1B [p Thr1265] Antibody - SuperBUGS](https://images.novusbio.com/images/MAP1B-[p-Thr1265]-Antibody-SuperBUGS-Western-Blot-NBP1-42827-img0005.jpg)
![Western Blot MAP1B [p Thr1265] Antibody - SuperBUGS](https://images.novusbio.com/images/MAP1B-[p-Thr1265]-Antibody---SuperBUGS-Western-Blot-NBP1-42827-img0003.jpg)
![Immunohistochemistry MAP1B [p Thr1265] Antibody - SuperBUGS](https://images.novusbio.com/images/MAP1B-[p-Thr1265]-Antibody---SuperBUGS-Immunohistochemistry-NBP1-42827-img0004.jpg)
| Publication using NBP1-49924 | Applications | Species |
|---|---|---|
| Johnson-Kerner BL, Garcia Diaz A, Ekins S, Wichterle H. Kelch Domain of Gigaxonin Interacts with Intermediate Filament Proteins Affected in Giant Axonal Neuropathy. PLoS One 2015-01-01 [PMID: 26460568] (IP) | IP |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
ICC | Human | 06/14/2023 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49924 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49923, which recognizes an upstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Immunoprecipitation-NBP1-49924-img0001.jpg "Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49924 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49923, which recognizes an upstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. GIG pulls down overexpressed PRPH in a Kelch-dependent manner. Cell lysates were subjected to immunoprecipitation with anti-Flag beads or IgG, and the membrane was probed sequentially for PRPH then GIG (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Immunoprecipitation-NBP1-49924-img0003.jpg "Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. GIG pulls down overexpressed PRPH in a Kelch-dependent manner. Cell lysates were subjected to immunoprecipitation with anti-Flag beads or IgG, and the membrane was probed sequentially for PRPH then GIG (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - Transfection with wild type Gigaxonin. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-49924_rabbit-polyclonal-gigaxonin-antibody-immunocytochemistry-immunofluorescence-196202316639..jpg "Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - Transfection with wild type Gigaxonin. Image from verified customer review.")
![Western Blot: Gigaxonin Antibody [NBP1-49924] - Design of proteomic bait to determine the binding partners of GIG.(A) GIG is a BTB/Kelch protein, & these domains are separated by an intervening BACK domain. GIG is thought to dimerize through its BTB domain [16]. The BTB domain has also been shown to bind to E3 ligases, potentially for ubiquitin tagging of Kelch targets. To identify potential substrates for degradation/modification (Kelch binding partners) we expressed flag-tagged full-length GIG (B) or its BTB domain (C). (D) Sequence of proteomic baits with key regions identified by color. The constructs expressed included a single flag tag & linker (grey), Biotag (black), BTB domain (blue), BACK domain (red), & Kelch domains (green). The location of the truncation for the BTB bait shown in (C) is indicated by the black line at amino acid 268, towards the N-terminus of the BACK domain. (E) Flag-tagged constructs were expressed in HEK293 cells & affinity purified with an anti-Flag antibody (abbreviated as F). The membrane was then probed with anti-Flag tag antibodies. Untransfected cells (NC) were included to control for non-specific binding to the antibody or beads. Flag-tagged GIG was ~62 kDa, while the BTB domain was ~34 kDa & forms a dimer in the immunoprecipitation sample that was ~70 kDa. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26460568), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-49924_rabbit-polyclonal-gigaxonin-antibody-310202416163788.jpg "Western Blot: Gigaxonin Antibody [NBP1-49924] - Design of proteomic bait to determine the binding partners of GIG.(A) GIG is a BTB/Kelch protein, & these domains are separated by an intervening BACK domain. GIG is thought to dimerize through its BTB domain [16]. The BTB domain has also been shown to bind to E3 ligases, potentially for ubiquitin tagging of Kelch targets. To identify potential substrates for degradation/modification (Kelch binding partners) we expressed flag-tagged full-length GIG (B) or its BTB domain (C). (D) Sequence of proteomic baits with key regions identified by color. The constructs expressed included a single flag tag & linker (grey), Biotag (black), BTB domain (blue), BACK domain (red), & Kelch domains (green). The location of the truncation for the BTB bait shown in (C) is indicated by the black line at amino acid 268, towards the N-terminus of the BACK domain. (E) Flag-tagged constructs were expressed in HEK293 cells & affinity purified with an anti-Flag antibody (abbreviated as F). The membrane was then probed with anti-Flag tag antibodies. Untransfected cells (NC) were included to control for non-specific binding to the antibody or beads. Flag-tagged GIG was ~62 kDa, while the BTB domain was ~34 kDa & forms a dimer in the immunoprecipitation sample that was ~70 kDa. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26460568), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Simple Western Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]](https://images.novusbio.com/images2/Serpin_A1_MAB1268_Immunocytochemistry__Immunofluorescence_17494.jpg)
![Immunocytochemistry Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]](https://images.novusbio.com/images2/Serpin_A1_MAB1268_Immunocytochemistry_11973.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
