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Tubulin alpha-8 Antibody (OTI2G6)

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Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - WB analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin alpha-8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-8.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded prostate tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-8 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded Kidney tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded pancreas tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunoprecipitation: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
OTI2G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

Order Details

Tubulin alpha-8 Antibody (OTI2G6) Summary

Immunogen
Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBA8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:150
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation
  • Western Blot 1:2000
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tubulin alpha-8 Antibody (OTI2G6)

  • Alpha-tubulin 8
  • TUBA8
  • TUBAL2
  • TUBAL2Tubulin alpha chain-like 2
  • tubulin alpha-8 chain
  • Tubulin alpha-8
  • tubulin, alpha 8

Background

Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TUBA8
Entrez