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Transcription factor E3 Antibody (TFE3/6849R) - Azide and BSA Free

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IHC analysis of formalin-fixed, paraffin-embedded human MiT translocation RCC. Nuclear staining using Transcription factor E3 antibody (TFE3/6849R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, ...read more
IHC analysis of formalin-fixed, paraffin-embedded human clear cell RCC. Negative tissue control using Transcription factor E3 antibody (TFE3/6849R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. ...read more
IHC analysis of formalin-fixed, paraffin-embedded human MiT translocation RCC. Nuclear staining using Transcription factor E3 antibody (TFE3/6849R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
TFE3/6849R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Transcription factor E3 Antibody (TFE3/6849R) - Azide and BSA Free Summary

Description
Positive Controls: MiT family translocated renal cell carcinoma. Ubiquitous mainly nuclear expression.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human Transcription factor E3 protein
Localization
Nucleus.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
TFE3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Transcription factor E3 Antibody (TFE3/6849R) - Azide and BSA Free

  • BHLHE33
  • bHLHe33transcription factor E3
  • Class E basic helix-loop-helix protein 33
  • RCCP2
  • TFEA
  • transcription factor binding to IGHM enhancer 3
  • transcription factor E family, member A
  • Transcription factor for IgH enhancer

Background

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TFE3