| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human TOB1 Met1-Asn345 Accession # P50616 |
| Specificity | Detects endogenous human TOB1 in Western blots. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TOB1 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
TOB1 (transducer of ErbB2 protein 1) is a 45 kDa member of the BTG family of anti‑proliferative proteins. It is ubiquitously expressed, and serves to negatively regulate cell cycle progression from G0/G1 to S phase. This is accomplished by TOB1 suppression of cyclin D1 expression, which is abrogated by Erk2-mediated phosphorylation of Ser152/154/164 in response to growth factor stimulation. Human TOB1 is 345 amino acids (aa) in length and contains one NES (aa 2‑14), a bipartite NLS (aa 18‑40), a BTG domain (aa 42‑130) that possesses antiproliferative activity, and two PAM domains (aa 133‑143 and 268‑278) that bind inducible PABP and regulate gene transcription. Human and mouse TOB1 (aa 1‑345) are 94% aa identical when fourteen mouse-specific glutamines are excluded from the alignment.
Secondary Antibodies |
Isotype Controls |
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