![Immunocytochemistry/Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050] - staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunocytochemistry-Immunofluorescence-NBP2-59050-img0011.jpg "Immunocytochemistry/Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050] - staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
| Reactivity | Hu, Mu, Fe, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clone | CL4455 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:PHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSG |
| Predicted Species | Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SOX10 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Feline | 03/14/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SOX10 |
![Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050] - Feline adult spinal cord demonstrating SOX10 (red) and DAPI (blue). Antibody at 1:200 dilutiion in 1X PBS + 0.05% tween 20+ 2% BSA. Tested at 1:50, 1:100, 1:200, 1:500, and 1:1000. Dilution of 1:200 gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with Alexa Fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Frozen-NBP2-59050-img0013.jpg "Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050] - Feline adult spinal cord demonstrating SOX10 (red) and DAPI (blue). Antibody at 1:200 dilutiion in 1X PBS + 0.05% tween 20+ 2% BSA. Tested at 1:50, 1:100, 1:200, 1:500, and 1:1000. Dilution of 1:200 gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with Alexa Fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy. Image submitted by a verified customer review.")
![Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-NBP2-59050-img0002.jpg "Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.")
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0004.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.")
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0005.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0009.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.")
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0007.jpg "Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
