Home » Siglec-3/CD33 » Siglec-3/CD33 Antibodies » Siglec-3/CD33 Antibody (Hu9a) [CoraFluor™ 1]
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Siglec-3/CD33 Antibody (Hu9a) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
Hu9a
Clonality
Monoclonal
Host
Human
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB9635U-100UG
Alexa Fluor 405
FAB9635V-100UG
Alexa Fluor 488
FAB9635G-100UG
Alexa Fluor 532
FAB9635X
Alexa Fluor 594
FAB9635T-100UG
Alexa Fluor 647
FAB9635R-100UG
Alexa Fluor 700
FAB9635N-100UG
Alexa Fluor 750
FAB9635S-100UG
Allophycocyanin
FAB9635A
Allophycocyanin/Cy7
FAB9635APCCY7
Biotin
FAB9635B-100
DyLight 350
FAB9635D
DyLight 405
FAB9635E
DyLight 488
FAB9635K
DyLight 550
FAB9635L
DyLight 594
FAB9635M
DyLight 650
FAB9635W
DyLight 680
FAB9635Y
DyLight 755
FAB9635Z
FITC
FAB9635F
HRP
FAB9635H
Janelia Fluor 525
FAB9635JF525
Janelia Fluor 549
FAB9635I
Janelia Fluor 585
FAB9635JF585
Janelia Fluor 635
FAB9635JF635
Janelia Fluor 646
FAB9635J
Janelia Fluor 669
FAB9635JF669
PE
FAB9635P
PE/Atto594
FAB9635PEATT594
PE/Cy5.5
FAB9635PECY55
PE/Cy7
FAB9635PECY7
PerCP
FAB9635C
Unconjugated
MAB9635-100
mFluor Violet 450 SE
FAB9635MFV450
mFluor Violet 500 SE
FAB9635MFV500
mFluor Violet 610 SE
FAB9635MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB9635-100
Carrier Free
FAB9635B-100
Carrier Free
MAB9635-100
Carrier Free
MAB9635-SP
Carrier Free
FAB9635G-100UG
Carrier Free
FAB9635V-100UG
Carrier Free
FAB9635U-100UG
Carrier Free
FAB9635T-100UG
Carrier Free
FAB9635S-100UG
Carrier Free
FAB9635R-100UG
Carrier Free
FAB9635N-100UG
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Siglec-3/CD33 Antibody (Hu9a) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody For Research Use Only
Immunogen
Human Siglec‑3/CD33
Specificity
Detects human Siglec‑3/CD33 based on Gemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Gemtuzumab. This product is for research use only.
Isotype
IgG1
Clonality
Monoclonal
Host
Human
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Siglec-3/CD33 Antibody (Hu9a) [CoraFluor™ 1]

  • CD33 antigen (gp67)
  • CD33 antigen
  • CD33 molecule
  • CD33
  • FLJ00391
  • gp67
  • myeloid cell surface antigen CD33
  • p67
  • sialic acid binding Ig-like lectin 3
  • Sialic acid-binding Ig-like lectin 3
  • Siglec3
  • Siglec-3
  • SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Siglec-3/CD33 Antibody (Hu9a) [CoraFluor™ 1]
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