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RFX2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: RFX2 Antibody [NBP2-13224] - Analysis in human testis and liver tissues. Corresponding RFX2 RNA-seq data are presented for the same tissues.
Western Blot: RFX2 Antibody [NBP2-13224] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: RFX2 Antibody [NBP2-13224] - Staining of human cell line MCF7 shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RFX2 Antibody [NBP2-13224] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: RFX2 Antibody [NBP2-13224] - Staining of human fallopian tube shows weak nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RFX2 Antibody [NBP2-13224] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: RFX2 Antibody [NBP2-13224] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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RFX2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: VMGEFNDLASLSLTLLDKDDMGDEQRGSEAGPDARSLGEPLVKRERSDPNHSLQGI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RFX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RFX2 Protein (NBP2-13224PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RFX2 Antibody

  • DNA binding protein RFX2
  • DNA-binding protein RFX2
  • FLJ14226
  • HLA class II regulatory factor RFX2
  • Regulatory factor X 2
  • regulatory factor X, 2 (influences HLA class II expression)
  • trans-acting regulatory factor 2

Background

RFX2 is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RFX2