R&D Systems Recombinant SARS-CoV-2 Spike (10549-CV) binds Recombinant Human ACE-2 (933-ZN) in a functional ELISA. The binding activity is approximately 4-fold greater than a top competitor’s Spike protein (full ...read more
2 μg/lane of Recombinant SARS-CoV-2 Spike His Protein (10549-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 144-175 ...read more
Recombinant SARS-CoV-2 Spike His Protein (Catalog # 10549-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (Catalog # 933-ZN) was measured at a concentration range between 0.37 nM ...read more
SDS-PAGE of 1 µg of R&D Systems Recombinant SARS-CoV-2 Spike (10549-CV) or a competitor’s Spike protein were run under reducing or non-reducing conditions and visualized by silver staining. The R&D Systems Spike ...read more
In a functional flow cytometry test, (A) Recombinant SARS-CoV-2 Spike His-tag Protein (Catalog # 10549-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and EGFP. Ligand ...read more
Recombinant SARS-CoV-2 Spike His Protein, CF Summary
Additional Information
Ectodomain, Stabilized Prefusion Conformation, Resistant to Furin Cleavage
Details of Functionality
Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag
(Catalog #
933-ZN).
Source
Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein Val16-Lys1211 (Arg682Ser, Arg685Ser, Lys986Pro and Val987Pro) with a C-terminal 6-His tag Suitable for use in serological assay development
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
134 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
144-175 kDa, under reducing conditions
Publications
Read Publications using 10549-CV in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant SARS-CoV-2 Spike His Protein, CF
2019-nCoV S Protein
2019-nCoV Spike
COVID-19 Spike
E2
Human coronavirus spike glycoprotein
Peplomer protein
S glycoprotein
S Protein
SARS-COV-2 S protein
SARS-COV-2 Spike glycoprotein
SARSCOV2 Spike protein
SARS-CoV-2
Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein
Spike glycoprotein
Spike
surface glycoprotein
Background
SARS-CoV-2,
which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs
to a family of viruses known as coronaviruses that are commonly comprised of
four structural proteins: Spike protein (S), Envelope protein (E), Membrane
protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein)
is a glycoprotein that mediates membrane fusion and viral entry. The S protein
is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and
S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the
S protein into the S1 and S2 subunits is required for activation. The S1
subunit is focused on attachment of the protein to the host receptor while the
S2 subunit is involved with cell fusion (3-5). The S protein of SARS-CoV-2
shares 75% and 29% amino acid (aa) sequence identity with the S protein of
SARS-CoV-1 and MERS, respectively.The S Protein
of the SARS-CoV-2 virus, like the SARS-CoV-1 counterpart, binds
Angiotensin-Converting Enzyme 2 (ACE2), but with much higher affinity and
faster binding kinetics through the receptor binding domain (RBD) located in
the C-terminal region of S1 (6). Based on structural biology studies, the RBD
can be oriented either in the up/standing or down/lying state with the up/standing
state associated with higher pathogenicity (7). Polyclonal antibodies to the
RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the
ACE2 receptor, confirming RBD as an attractive target for vaccinations or
antiviral therapy (8). It has been demonstrated that the S Protein can invade
host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (9, 10).
A SARS-CoV-2 variant carrying the S protein aa change D614G has become the most
prevalent form in the global pandemic and has been associated with greater
infectivity and higher viral load (11, 12).
Wu, F. et al. (2020) Nature 579:265.
Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93.
Bosch, B.J. et al. (2003). J. Virol. 77:8801.
Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
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