SARS-CoV-2 Spike Antibodies
The SARS-CoV-2 Spike (S) protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19. The SARS-CoV-2 Spike protein has a theoretical molecular weight of approximately 180 kDa and consists of a S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail. The viral Spike protein binds to the cell surface receptor angiotensin converting enzyme 2 (ACE2) resulting in fusion and virus entry into the cell. Browse our extensive catalog for SARS-CoV-2 Spike polyclonal antibodies, monoclonal antibodies including human monoclonal and rabbit monoclonal antibodies, and fluorescently-conjugated 2019-nCoV Spike antibodies. Validated research applications for SARS-CoV-2 Spike antibodies include Block/Neutralize, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Paraffin, and Western Blot. Choose the viral-specific anti-SARS-CoV-2 Spike antibody that is best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each SARS-CoV-2 Spike (S) antibody is fully covered by our Guarantee+.
Alternate Names for SARS-CoV-2 Spike Antibodies
anti-2019-nCoV S Protein antibody, anti-2019-nCoV Spike antibody, anti-COVID-19 Spike antibody, anti-Human coronavirus spike glycoprotein antibody, anti-S Protein antibody, anti-SARS-COV-2 S protein antibody, anti-SARS-COV-2 Spike glycoprotein antibody, anti-SARSCOV2 Spike protein antibody, anti-SARS-CoV-2 antibody, anti-Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein antibody, anti-Spike glycoprotein antibody, anti-Spike antibody
30 Results for "sars-cov-2-spike" in Primary Antibodies
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Validation of SARS-Cov-2 Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody NBP3-11940, 0.2-1 ug/mL incubated at RT for 1 hr. Secondary Antibody: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. NBP3-11940 can detect spike S1 protein for all variants except omicron.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0009.jpg "ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Validation of SARS-Cov-2 Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody NBP3-11940, 0.2-1 ug/mL incubated at RT for 1 hr. Secondary Antibody: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. NBP3-11940 can detect spike S1 protein for all variants except omicron.")
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-11940] - Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike (cleavage site) in 293 Transfected Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Spike transfected 293cells labeling SARS-CoV-2 (COVID-19) Spike (cleavage site) with NBP3-11940 at 10 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11940-img0005.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike, NBP3-11940 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0006.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Western Blot Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0007.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Detection of SARS-CoV-2 UK Variant Spike S1 Protein with Spike S1 Antibodies by Direct ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 ug/mL Incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody, NBP3-11940, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody NBP3-11940 includes site 681P that was mutated in B.1.1.7.. SARS-CoV-2 Spike antibody (NBP3-11940) has lower binding affinity for variant S1 protein as compared to WT S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation of SARS-CoV-2 Spike Antibody Pair. A sandwich ELISA was performed using the anti-SARS-CoV-2 Spike antibodies NBP3-11940 (10 ug/mL ) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies (1 ug/mL ) and streptavidin-HRP (0.1 ug/mL ) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein, as coating antigen and the anti-SARS-CoV-2 Spike as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Antibodies: SARS-CoV-2 Spike antibody, NBP3-11940 (1 ug/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP2-41058] - Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. SARS-CoV-2 Spike(1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP2-41058-img0001.jpg "Western Blot: SARS-CoV-2 Spike Antibody [NBP2-41058] - Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. SARS-CoV-2 Spike(1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells")
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike Antibody [NBP2-41058] - Immunohistochemistry Validation of SARS-CoV-2 Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 Spike S2 antibody (NBP2-41058, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunohistochemistry-Paraffin-NBP2-41058-img0003.jpg)
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike Antibody [NBP2-41058] - Immunohistochemistry Validation of SARS-CoV-2 Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 Spike S2 antibody (NBP2-41058, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunohistochemistry-Paraffin-NBP2-41058-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90979] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022) . For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90979-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90979] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022) . For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90979] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90979-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Examination of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7) UK, beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK can specifically detect alpha variant and Mu spike S1 protein, but not WT and other tested variants.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11931-img0003.jpg "ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Examination of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7) UK, beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK can specifically detect alpha variant and Mu spike S1 protein, but not WT and other tested variants.")
![Western Blot: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Validation of UK Variant Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-Western-Blot-NBP3-11931-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - ELISA Validation of UK Variant Spike Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody NBP3-11931 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11931-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Validation of SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) with Mutant and WT Spike S1 Proteins. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11931-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody Alpha Variant (B.1.1.7) UK NBP3-11941, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11941 can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11941-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody Alpha Variant (B.1.1.7) UK NBP3-11941, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11941 can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.")
![Western Blot: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11941, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11941) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-Western-Blot-NBP3-11941-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11941, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11941) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11941-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11941, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11941) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11941-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-ELISA-NBP3-12856-img0003.jpg "ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, NBP3-12856, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-Western-Blot-NBP3-12856-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, NBP3-12856, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-ELISA-NBP3-12856-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - ELISA Validation of Gamma Variant Spike S1 Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT and Gamma variant (P.1), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, NBP3-12856, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma variant spike S1 peptide (26S), but not WT peptide (26P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-ELISA-NBP3-12856-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, NBP3-12854, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 alpha variant spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7A4D12-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-12854-img0003.jpg "ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, NBP3-12854, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 alpha variant spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.")
![Western Blot: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, NBP3-12854, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7A4D12-Alpha-Variant-B-1-1-7-UK-Western-Blot-NBP3-12854-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, NBP3-12854, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7A4D12-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-12854-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, NBP3-12854, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7A4D12-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-12854-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (1C3H9) [NBP3-12855] - SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, NBP3-12855, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-1C3H9-ELISA-NBP3-12855-img0003.jpg "ELISA: SARS-CoV-2 Spike Antibody (1C3H9) [NBP3-12855] - SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, NBP3-12855, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody (1C3H9) [NBP3-12855] - ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, WT and Gamma variant (P.1). Detection Antibodies: Spike 26P antibody, NBP3-12855, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein, but not Gamma variant spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-1C3H9-Western-Blot-NBP3-12855-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (1C3H9) [NBP3-12855] - ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins of WT and Gamma variant (P.1), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, NBP3-12855, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein, but not Gamma variant spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-1C3H9-ELISA-NBP3-12855-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (1C3H9) [NBP3-12855] - ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT and Gamma variant (P.1), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, NBP3-12855, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 spike 26P antibody detects WT peptide (26P), but not Gamma variant spike S1 peptide (26S).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-1C3H9-ELISA-NBP3-12855-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - Validation of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike Antibody alpha variant (B.1.1.7) UK NBP3-11942, dilution: 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11942 can specifically detect omicron and alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11942-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - Validation of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike Antibody alpha variant (B.1.1.7) UK NBP3-11942, dilution: 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11942 can specifically detect omicron and alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.")
![Western Blot: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-Western-Blot-NBP3-11942-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11942-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11942-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14793, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7E3C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14793-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14793, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.")
![ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7E3C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14793-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, NBP3-14793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R), but not WT peptide (681P) and Alpha variant spike S1 peptide (681H).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7E3C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14793-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14792, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5H4C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14792-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14792, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.")
![ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14792, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5H4C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14792-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, NBP3-14792, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R), but not WT peptide (681P) and Alpha variant spike S1 peptide (681H).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5H4C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14792-img0003.jpg)