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PUS3 Antibody

Images

 
Western Blot: PUS3 Antibody [NBP2-55852] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: PUS3 Antibody [NBP2-55852] - Staining of human cell line PC-3 shows localization to nucleoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PUS3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DLRSQFPRGRDSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PUS3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PUS3 Recombinant Protein Antigen (NBP2-55852PEP)

Reactivity Notes

Mouse 86%, Rat 82%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PUS3 Antibody

  • EC 5.4.99.-
  • FKSG32
  • FLJ23638
  • pseudouridylate synthase 3,2610020J05Rik
  • tRNA pseudouridine synthase 3
  • tRNA pseudouridylate synthase 3
  • tRNA-uridine isomerase 3

Background

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PUS3