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OXSR1 Overexpression Lysate

Name: OXSR1 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-14025
Price: 679 USD

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Western Blot: OXSR1 Overexpression Lysate (Adult Normal) [NBL1-14025] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OXSR1.

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB

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OXSR1 Overexpression Lysate Summary

Description

OXSR1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC200396

Accession#: NM_005109

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Gene

OXSR1

Applications/Dilutions

Dilutions	Western Blot
Application Notes	This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW	57.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

Lysate Details for Array

Type

Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for OXSR1 Overexpression Lysate

EC 2.7.11
EC 2.7.11.1
KIAA1101OSR1serine/threonine-protein kinase OSR1
Oxidative stress-responsive 1 protein
oxidative-stress responsive 1

Background

The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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OXSR1
Overexpression Lysate

NBL1-14025

Species: Hu

Applications: WB

Publications for OXSR1 Lysate (NBL1-14025) (0)

There are no publications for OXSR1 Lysate (NBL1-14025).

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Product General Protocols

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Video Protocols

WB Video Protocol

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Protein Kinase

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Bioinformatics

Gene Symbol

OXSR1

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

OXSR1 Overexpression Lysate

Species Glossary

Images

Product Details

Order Details

OXSR1 Overexpression Lysate Summary

OXSR1 Transient Overexpression Lysate

Applications/Dilutions

Packaging, Storage & Formulations

Lysate Details for Array

Notes

Alternate Names for OXSR1 Overexpression Lysate

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for OXSR1 Lysate (NBL1-14025) (0)

Reviews for OXSR1 Lysate (NBL1-14025) (0)

Product General Protocols

Video Protocols

FAQs for OXSR1 Lysate (NBL1-14025) (0)

Additional OXSR1 Products

Research Areas for OXSR1 Lysate (NBL1-14025)

Blogs on OXSR1

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Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics